170 



STATE BOARD OF AGRICULTURE. 



6 HR. 45 CC. VIRUS NaCl. 



Immune pig No. 336, wt. 120 lbs., was hjpenmmunized by the quick 

 subcutaneous method bv injecting 10 cc. per lb. of body wt. of G hr. 45 

 CC. virus NaCl from virus pig No. 4G1-B. 



Autopsy of viiTis pig No. IGl-B : Kidneys congested. Lymph glands 

 enlarged with cortex hemorrliagic. Lungs hemorrhagic. 'Hpleen enlarged 

 and hemorrhagic. Heart normal. 



Hyper No, 33G was bled 3 times, then killed, 4195 cc. of serum being 

 secured and tested as follows: 



5 HOUR 30 CC.-7 HOUR 24 cc.-7 HOUR 30 cc.-ll HOUR 26 cc. VIRUS NaCI. 



Immune pig No. 277 was hyperimmunized as follows : 



G-20-'ll — Injected subcutaneously G50 cc. 5 hr. 30 cc. virus NaCl from 

 virus pigs Nos. 290-291. 



Autopsy of virus pig No. 290: Few petechiae on kidneys. Lungs 

 hemorrhagic. Liver congested. Spleen enlarged. Lymph glands en- 

 larged and hemon-hagic. Intestines congested. 



Autopsy of virus pig No. 291 : Lungs hemorrhagic. Kidneys con- 

 gested. Inguinal and sublumbal glands enlarged and congested. 



7-3-'ll — Injected subcutaneously 1200 cc. 11 hr. 26 cc. virus NaCl from 

 virus pig No. 408. 



Autopsy of viims pig No. 408: Few petechiae on kidne3\s. Liver con- 

 gested. Lymph glands hemorrhagic. Spleen enlarged. Heart normal. 

 Lungs hemorrhagic. 



7-7-'ll — Injected subcutaneously 1850 cc. 7 hr. 30 cc. virus NaCl from 

 virus pig No. 286, making a total injection of 18 cc. per lb. of body wt. 



Autopsy of virus pig No. 286 : Congested lymph glands. Spleen en- 

 larged and hemorrhagic. Few petechiae on kidneys. Lungs and heart 

 normal. 



This hyperimmune was bled 3 times from tail, then re-hyperimmunized 

 by injecting subcutaneously 8 cc, per lb, of body wt. of 7 hr, 24 cc, vinis 

 NaCl from vinis pig No, 346. 



Autopsy of virus pig No. 346 : Congested lymph glands. Few pete- 

 chiae on kidneys. Spleen enlarged and hemorrhagic. Lungs hemorr- 

 hagic. Heart normal. 



After re-hvperimmunizing tliis hvper. was bled 3 times from tail, then 

 killed. 



Autopsy: All organs normal and virus well absorbed, A total of 

 8715 cc. of seinim was secured, which was tested as follows : 



