EXPERIMENT STATION REPORTS. 



171 



5 HOUR 30Icc.-81HOUR:22Icc.-6IHOURI;25:cc.-7 HOUR 30 CC.-6 HOUR 40 cc. - VIRUS NaCl. 



Immune pig No. 282, wt. 232 Ibfs., was hyperimmimized as follows: 



6-20-'ll — Injected subcntaneonsly 650 cc. 5 hr. 30 cc. virus NaCl from 

 virus pigs Nos. 290-201. (See autopsies above.) 



7-5-'ll — Injected subcutaneously 2000 cc. 8 hr. 22 cc. virus NaCl from 

 virus pig No. 402. 



Autopsy of virus pig No. 402 : Few petechiae on kidneys. Spleen en- 

 larged and hemorrhagic. Lymph glands hemorrhagic, 



7-7-'ll — Injected subcutaneously 700 cc. 7^/^ hr. 30 cc. virus NaCl 

 from virus pig No. 289, this making a total injection of 141/0 cc. per lb. 

 of body wt. 



Autopsy of virus pig No. 289 : Few petechiae on kidneys. Spleen -en- 

 larged and hemorrhagic. Intestines ulcerated. Lungs hemorrhagic. 

 Liver congested. 



This hyperimmune was bled 3 times from arteries of tail, then re- 

 hyperimmunized as follows: 



8-24-'ll — Injected subcutaneously 600 cc. 5 hr. 30 cc. virus NaCl from 

 viiiis pig No. 422- A, 



Autopsy of virus pig No. 422-A: Numerous i)etechiae on kidneys. 

 Lymph glands enlarged and congested. Spleen enlarged and containing 

 hemorrhagic areas. 



8-31-'ll — Injected subcutaneously 1200 cc. 7 hr. 30 cc. virus NaCl from 

 virus pig No. 423-b. Thus, in re-hyperimmunizing, 8 cc. per lb. of body 

 wt. was injected. 



Autopsy of virus pig No. 423. (See above.) 



After re-hyj3erimmunizing, hyer. No. 282 was bled 3 times from tail, 

 then killed, 8750 cc. of serum being secured. On autopsy, this animal 

 showed all organs normal. No abscesses were formed, however tissue 

 at points of injection appeared inactive, metabolism not being carried 

 on normallv. The serum secured was tested as follows: 



