172 



STATE BOARD OP AGRICULTURE. 



5 HOUR 30 CC.-7* HOUR 30 CC." VIRUS NaCl. 



Immime pig No. 283, wt. 200 lbs., was hyperlmmiinized as follows: 



6-20-'ll — Injected siibcntaneoiisly 700 re. 5 hr. 30 cc. virus NaCl from 

 virus pigs Nos. 290-291. (See above autopsies.) 



7-5-'ll — Injected subcutaneously 2000 cc. Ti/o hr. 20 cc. virus NaCl 

 from virus pig No. 404. 



Autopsy of virus pig No. 404 : Numerous petechiae on kidneys. Lymph 

 glands enlarged with cortex lieniorrhagic. Spleen enlarged and hemorr- 

 hagic. Lungs hemorrhagic. Heart normal. 



7-7-'ll — Injected subcutaneously 700 cc. 7yo hr. 30 cc. virus NaCl from 

 vims pig No, 2S9, (see autoijsy above), making a total injection of I6V2 

 cc. ])er lb. of body wt. 



This hyper, was bled 3 times from tail, then re-hypenmnuinized by in- 

 jecting 71/2 cc. per lb. of body wt. of 6I/2 hr. 30 cc. virus NaCl from virus 

 pig No. 415. 



Autopsy of virus pig No. 41.5: Lungs hemorrhagic. Liver and spleen 

 congested, the latter being enlarged. Lymph glands enlarged and 

 hemorrhagic. Intestines ulcerated. Few petechiae on kidneys. After 

 re-hyperimmunizing, hyper. No. 283 was bled 3 times from tail, then 

 killed. A total of 9160 cc. of serum was secured and tested as follows: 



5 HOUR 30 CC.-6 HOUR 25 cc.-8 HOUR 22 CC. VIRUS NaCl. 



Immune pig No. 284, wt. 230 Ibi*., was hyperimmunized as follows: 



6-20-'ll — Injected subcutaneously 700 cc. 5 hr. 30 cc. virus NaCl from 

 ^^rus pigs Nos. 290-291. (See above autopsies.) 



7-o-'ll — Injected subcutaneously 2400 cc. 8 hr. 22 cc.-O hr. 25 cc. virus 

 NaCl from virus pigs Nos. 403-402. 



Autopsy of virus pig No. 403 : Few petechiae on kidneys. Lymph 

 glands enlarged and hemorrhagic. Spleen abnormal in size and con- 

 taining numerous hemorrhagic areas. Heart and lungs normal. 



This hyperimmune was bled 3 times from tail, then re-hyperimmunized 

 by injecting 1600 cc. 6 hr. 36 cc. virus NaCl from virus pig No. 416. 



Autopsy of virus No. 416: Lymph glands enlarged and congested. 

 Few petechiae on kidneys. Spleen enlarged. Intestines congested. 

 Lungs and heart normal. 



After re-hyperimmunizing, this pig was bled 3 times from tail, then 

 killed. Autopsy showed all organs normal. A total of 9100 cc, of serum 

 was secured, which was tested as follows: 



