EXPERIMENT STATION REPORTS. 



173 



5 HOUR 30 CC.-7 HOUR 23 CC.-7i HOUR 30 CC. VIRUS NaCL 



Immune pig No. 285, wt. 228 lbs., was hyperimmunized as follows: 



6-20-'ll — Injected subcutaneously 700 cc. 5 hr. 30 cc. virus NaCl from 

 virus pigs Nos. 290-291. (See above autopsies.) 



7-5-'ll — Injected subcutaneously 2000 cc. 7 hr. 23 cc. virus NaCl from 

 virus pigs Nos. 406b and 407. 



Autopsy of virus pig No. 406 : Numerous petechiae on kidneys. Lungs 

 hemorrhagic. Liver and spleen congested. Lymph glands congested. 



Autopsy of virus pig No. 407 : Numerous petechiae on kidneys. Spleen 

 enlarged and hemorrhagic. Lymph glands hemorrhagic. Liver con- 

 gested. Heart and lungs nonnal. 



7-7-'ll — Injected subcutaneously 700 cc. 71/2 hr. 30 cc. virus NaCl from 

 viinis pig No. 289. (See above autopsy.) Thif? mates a total injection 

 of 141/2 cc. per lb. of body w^t. 



This hyperimmune w^as bled 3 times from tail, then re-hyperimmunized 

 as follows: 



8-10-'ll — Injected subcutaneously 650 cc. 7 hr. 30 cc. virus NaCI from 

 virus pig No. 418. 



Autopsy of virus pig No. 418: Few petechiae on kidneys. Lymph 

 glands enlarged and congested. Spleen enlarged and congested. Heart 

 and lungs normal. 



8-24-'ll — Injected subcutaneously 1200 cc. 5 hr. 30 cc. virus NaCl from 

 virus pig No. 422. (See above autopsy.) Thus making a total injection 

 of 8 ec. per lb. of body wt. in re-hyperimmunizing. 



After re-hyperimmunizing, hyper No. 285 was bled 3 times from tail, 

 then killed. Autopsy showed all organs normal. 9465 cc. of serum were 

 secured and tested as follows: 



