174 



STATE BOARD OF AGRICULTURE. 



6 HOUR 30 cc.-7i HOUR 30 cc. VIRUS NaCl. 



Immune pig No. 310, wt. 98 lbs., was hyperimmunized as follows: 



9-12-'ll — Injected subcutaneously 800 cc. G hr. 30 cc. virus NaCl from 

 virus pig No. 384. (See above autopsy.) 



10-9-'ll — Injected subcutaneously 670 cc. 6 hr. 30 cc. virus NaCl from 

 virus pig No. 431, making a total injection of 15 cc. i)ev lb. of body wt. 



This hyijerimmune was bled 3 times from tail, then killed, 3200 cc. 

 of serum being secured, which was tested as follows : 



6 HOUR 33 CC.-6 HOUR 36 CC-TJ HOUR 39 CC. VIRUS NaCl. 



Immune pig No. 303, wt. 170 lbs., was hyperimmunized as follows: 



9-8-'ll — Injected subcutaneously 900 cc. G hr. 33 cc. virus NaCl from 

 virus pig No. 341. 



Autopsy of virus pig No. 341 : Few petechiae on kidneys. Spleen 

 abnormal in size and containing hemorrhagic areas. Lymph glands 

 enlarged and congested. Heart and lungs normal. 



9-ll-'ll — Injected subcutaneously 1200 cc. 714 br. 39 cc. virus NaCl 

 from viinis pig No. 380. 



Autopsy of virus i>ig No. 380: Lungs hemorrhagic. Liver congested. 

 Intestines ulcerated slightly. Petechiae on cortex of kidneys and auricles 

 of heart. 



9-23-'ll — Injected subcutaneously 900 cc. mixture of G hr. 36 cc. and 

 7 hr. 38 cc. virus NaCl from virus pigs Nos. 432 and 435, respectively, 

 making a total injection of 18 cc. per lb. of body wt. 



Autopsy of virus pig No. 432: Few petechiae on kidneys. Spleen 

 enlarged and congested. Lymph glands enlarged and hemorrhagic. 

 Liver normal. Lungs hemorrhagic. 



Autopsy of virus pig No. 435 : No petechiae on kidneys, but congested. 

 Lymph glands congested. Spleen enlarged and hemorrhagic. Heart and 

 lungs normal. Intestines dotted with petechiae. 



This hyi)erimmune was bled 3 times from the tail, then re-hyperim- 

 munized by injecting subcutaneously 1500 cc. of mixture of G hr. 30 cc. 

 and G hr. 40 cc. virus NaCl from virus pigs Nos. 431 and 438. 



Autopsy of virus pig No. 438: Few petechiae on kidneys. Lymph 

 glands enlarged and congested. Small ulcers on intestines. Spleen en- 

 larged and hemorrhagic. Heart and lungs normal. 



Hyper No. 303 was bled 3 times after re-hyperimmunizing and killed. 

 A total of 8455 cc. of serum was secured which was tested as follows: 



