EXPERIMENT STATION REPORTS. 175 



Dat«. J^ "^,4^^^- Material iajected. Result of test. 



No. (.Lbs.) 



ll-lsr-ll 4S; 115 20 cc. serum 



2 oc. Tirus Lived. 



11-lS-ll 483 101 ! 15 cc. senim j 



; I 2 cc. Tims lived. 



11-lS-ll ■4S4 105 10 cc serum 12-3-11, died. 



2 cc virus . . . Autopsy: Cholera lesions. 



11-lS-ll 4S5 101 2 cc. virus. ... 12-6-11, killed. 



I Autopsy: Cholera lesions. 



I 



6 HOITR 42 CC.-7 HOUR 38 cc. TIRUS XaCl 



Immune pig ^o. 312. wt. 145 lbs., was hvfierimmunized as follows: 



9-14-"ll — Injected subcutaneouslv 1400 cc. 6 hr. 42 cc. virus XaCl from 

 virus pig Xo. 422b. 



AuTof»ST of virus pig Xo. 422b: Numerous petechiae on kidneys. 

 Lvmph glands enlarged and congested. Lungs and heart normal. Spleen 

 enlai'ged and congested. Intestines congested. 



9-23-'ll — Injected subcutaneouslv 1600 cc. mixture of 6 hr. 36 cc. and 

 7 hr. 38 cc. virus XaCl fi\>m virus pigs Xos. 432-435. ( See above 

 autopsies •. making a total injection of 20 cc. per lb. of body wt. 



This hyj:»er was bled 3 times from tail, then re-hyperimmunized by 

 injecting subcutaneously 15CM} cc. mixture of <> hr. 40 cc. and 7 hr. 42 cc. 

 ZS'aCl from virus pigs Xos. 431b and 4oS. (See autoi;»sy of 43S.) 



Autopsy of virus pig No. 431b : Petechiae on -kidneys. Lymph glands 

 enlarged and congested. Spleen enlai-ged and hemorrhagic. Intestines 

 congested. 



Hyperimmune was bled a total of 7 bleedings, including killing. G007 

 cc. of serum being secured which was tested as follows: 



Date. Pi& Weight. Material injected. i Restilt of test. 



No. (Lrs. 



11-18-11 4S6 I 110 20 cc. serum. 



2 cc. virus. . 



11-18-11 4S7 104i 2 cc. virus.. 



15 cc. serum. 



11-18-11 4SS 112 lOcc. senun 



Lived. 



12-13-11, killed. 



Autopsy: Cholera lesions. 



12-12-11, died. 



2 cc. virus. . . Autopsy: Cholera lesions. 



11-18-11 4S5 101 , 2 cc. virus . .. 12-6-11. killed. 



Autopsy: Cholera lesions. 



7* HOm 40 cc. VIRUS XaQ. 



Immune pig Xo. 308. wt. 152 lbs., was hyperimumnized as follows : 



9-11 '11 — Injected subcutaneously 1000 cc. 7^2 hr. 39 cc. virus XaCl 

 from virus pig Xo. 380. (See above autopsy.) 



10-14-'ll — Injected subcutaneouslv 2000 cc. 7io hr. 40 cc. virus Zs'aCl 

 from virus pig Xo. 428. making a total injection of 20 cc. per lb. of 

 body wt. 



Autopsy of virus pig Xo. 428: Petechiae on kidneys. Lymph glands 

 enlarged and hemorrhagic. Spleen enlarged and hemorrhagic. Lungs 

 hemorrhagic. 



