EXPERIMENT STATION REPORTS. 



177 



No. of 

 pigs 

 used. 



2 

 6 

 2 

 1 

 10 

 2 



Rate of 



injection, 



per lb. 



25 cc. 

 25 cc. 

 30 cc. 

 45 cc. 

 30 cc. 

 40 cc. 

 45 cc. 



25 cc. 

 30 cc. 



33 cc. 

 36 cc. 

 40 cc. 



45 cc. 



27 cc. 

 30 cc. 



34 cc. 

 38 cc. 



40 cc. 



22 cc. 



24 cc. 



26 cc. 



25 cc. 



Time 



let 



remain. 



3 hrs. 



4 hrs. 

 4 hrs. 



4 hrs. 



5 hrs. 

 5 hrs. 



5 hrs. 



6 hrs. 

 6 hrs. 



6 hrs. 

 6 hrs. 

 6 hrs. 



6 hr.s. 



6i hrs. 



7 hrs. 



7 hrs. 



7 hrs. 



7i hrs. 



8 hrs. 



9 hrs. 

 11 hrs. 

 14 hrs. 



Average 



% of 



increase. 



183 



147 



142 



266 



199 



282 



306 



156 

 213 



125 

 205 

 309 



231 



255 

 161 



240 

 196 



455 



172 



102 



163 



36 



Value of virus NaCI. 



Produced sera of low potency when used at rate of 10 cc. 

 12i cc. per lb. of body wt. of immune. 



Produced potent sera when used at rate of 10 cc.-12i cc. 

 per lb. in hyperimmuiiizing. 



Produced potent sera when injected at rate of 10 cc. per lb 

 in hyperimmunizing. 



Produced sera of low potency when used at rate of 10 cc. 

 per lb. 



Produced very potent sera when injected at rate of 10 cc- 

 12 CC.-15 cc. per lb. in hyperimmunizing. 



Produced potent sera when used at rate of 10 cc. per lb. in 

 hyperimmunizing. 



Produced sera of low potency when used at rate of 10 cc. 

 per lb. in hyperimmunizing. 



Produced potent sera. 



Produced very potent sera when used at rate of 18cc. per 

 lb. in hyperimmunizing. 



Produced potent sera. 



Produced potent sera when used with other virus. 



Produced sera of low potency when used at rate of 10 cc. 

 per lb. in hyperimmunizing. 



Produced serum with fair potency when used at rate of 10 

 cc. per lb. - 



Produced potent sera . 



Produced very potent sera when used with other virus in 

 hyperimmunizing. 



Produced potent sera. 



Produced potent sera when used with other virus in hyper- 

 immunizing. 



Produced sera of low potency when used at rate of 15 cc. 

 per lb. in hyperimmunizing. 



Produced very potent sera when used together with other 

 virus. 



Produced potent sera when used together with other virus. 



Produced very potent sera. 



These data may be briefly summarized as follows: 



We found that the virulence of salt solution injected into the ab- 

 dominal cavity of virus pigs varies greatly with amount of injection as 

 well as time let remain. 



The per cent of injected solution recovered varies greatly with size 

 and age of pig as well as with time let remain in abdominal cavity. 



Salt solution injected into the abdominal cavity of virus pigs in 

 amounts not exce<j(ling 30 cc. per pound of body weight and let remain 

 not less Ihan five hours, or for a longer time, was found to be eflQcient 

 in hyperimmunizing animals. 



Since completing this experiment, I have hyperimmunized about 100 

 23 



