180 STATE BOARD OF AGRICULTURE. 



^ CULTURES. 



No. of cultures alfalfa sent out 3,134 



'So. of cultures red clover sent out 213 



No. of cultures alsike clover sent out 21 



No. of cultures white clover sent out 6 



No. of cultures soy beans sent out 137 



No. of cultures white beans sent out 10 



No. of cultures i>eas sent out 74 



No. of cultures cowpeas sent out 82 



No. of cultures sweet peas sent out 1 



No. of cultures vetch sent out 75 



3,753 



No. of cultures for which collections are outstanding 51 



No. of cultures sent gratis 26 



No. of alcohol-acetic cultures sent out 12 



No. of lactic cultures 2 



Dr. Giltner submits his experiences with avian tuberculosis and his 

 work as State Veterinarian. 



AVIAN TUBERCULOSIS. 



We have observed a number of new outbreaks of tuberculosis in chick- 

 ens during the jear. A lecture and demonstration Ijefore the short 

 course students in poultry resulted in disclosing this disease in several 

 places. 



Experimentally, we have not been able to accumulate very much data, 

 hence valuable results are not at hand. Mr. Himmelberger has suc- 

 .ceeded in cultivating the avian turbercle germ on sterile banana slants, 

 transfers being made from potato broth which had been inoculated from 

 I)otato slants. Avian tuberculin has been made and its action tested 

 in a limited manner by injection into the comb and by instillation into 

 the conjunctival sac of chickens without result. 



Our tests do not indicate that cohabitation surely or quickly results 

 in the transmission of the disease. Of seven white leghorn hens kept 

 in company with and later in uncleaned pens occupied by tuberculous 

 hens, none showed lesions of tuberculosis after several months. More 

 recent tests with healthy hens kept in intimate association with tuber- 

 culous birds in advanced stages of the disease fail to show transmission 

 of the disease after five weeks. We have also been unable to transmit 

 the disease to white rats kept in cages occupied by tuberculous birds 

 or by subcutaneous injections of pure cultures. These experiments are 

 being continued. 



We cannot report satisfactorily on the diagnosis of this disease by 

 microscopic examination of smears of fecal matter secured either by 



