EXPERIMENT STATION REPORTS. 



181 



swabs through the cloaca or from droppings. One case in particular 

 failed to show tubercle bacteria after such examination, although fatally 

 affected with the disease. An examination was made only four days 

 before death. 



Some apparent relationshii)s between the etiologic organism in Johne's 

 disease of cattle and that of avian tuberculosis have induced us to test 

 the effects of the avian germ on calves. On ^lay 17, 1911. twin calves 

 about two weeks old were fed in milk the finely chopped organs of a 

 tuberculous hen. The organs were seriously affected. This material 

 was entirely eaten only after two days. Slight diarrhea supervened and 

 rapidly disap)peared. On July 5th. the male calf died suddenly from 

 acute tympanites. No effects of the ingestion of tubercle germs could 

 be seen at autopsy. On July 16th and 17th, the female calf was tested 

 with avian tuberculin. A normal calf was also tested at this time. The 

 tuberculin was made by cultivating the avian tulwrcle genu in potato 

 broth for eight Meeks and evaporating to one-fifth its original volume. 

 The concentrated solution was preserved in .5 per cent phenol. 



The laboratory calf had a slight diarrhea during the reaction. At 

 2 p. m. on the next day, the temperature was l(i:>.2"\ We are unwilling 

 to sacrifice this calf for the purpose of verifying this test by autopsy, 

 but this will be done later. 



Twort and Ingram^ state that, "Animals suffering from pseudo-tuber- 

 culous enteritis, either normally contracted or experimentally produced 

 by the inoculation of pure cultures of Johne's bacillus, give no definite 

 reaction with diagnostic vaccines prepared from cultures of the timothy 

 grass bacillus or from avian tubercle bacillus.'' If our calf has really 

 developed lesions of tuberculosis as a result of ingestion of avian tuber- 

 cular material, then we have evidence against there being any relation 

 between the two diseases under discussion. 



WARD GILTNER. 



«. Proc. Roy. Soc. Ser. B. 84 (1912) No. B575. pp. 517-542. 



