— 307 — 



surface of the water is only seen a single fragment of a stem, having 

 also some fresh buds. On the microscopic examination of a knot the 

 resting spores appear fully unchanged. The starch in the knots is de- 

 generated. 



Novbr. 26. — The turions on the decaying stems fully fresh. The 

 resting spores essentially unchanged; their contents however is differen- 

 tiated in a more dense, parietal layer rich with oil and a homogeneous central 

 part of a less dense consistence. Rarely is seen an inwardly irregularly 

 limited thickening of the membrane, reaching upon the whole not more 

 than 2 n. On our later examination of the material at hand of the 

 Museum we have found that resting spores of this type only occur as a 

 rare exception — and a thickness of the membrane like that mentioned 

 by Rostrup — 4 // — has never appeared in our material. The placing 

 of the oil-drops in a parietal layer seems to be normal^). 



Dec. 21. — All the material sunk. The resting spores are fully 

 unchanged, having the aspect as shows fig. 1, a. The appendicular cells 

 with their remnants of hyphae (in a number of until 6) are still persisting 

 in many cases. 



1909 Jan. 15. — The resting spores unchanged. The thickness of 

 the membrane is on an average 1^/2 fx. The turions are looking fresh 

 and viable. The wall of the glass is covered with a thick layer of dia- 

 toms, which keep the water fresh. For this reason Racteria and Gyano- 

 phyceae are sparingly occurring. 



Febr. 10. — Resting spores unchanged, still with appendicular cells 

 in many cases. At this time the turions begin to stretch. The lower 

 leaves are being expanded and take up a much recurved position. 



March 4. — Germinating resting spores for the first time observed. 

 (In order to avoid giving a fragmentary picture of the germination we 

 prefer to report the whole progress under this date). On examining many 

 samples of our material at short intervals we have found that the spores 

 are germinating during a pair of months — March till the beginning of 

 May — according to the fact that the turions of Myriophyllum verticilla- 

 turn begin to stretch about the midst of February and are developing 

 side-branches towards the beginning of May. 



In a very few cases we have observed a germination by hyphae, 

 as it is pictured in fig. 1, c and d. The hyphae, which go out from 



The same character occurs in Cladoehytrinm Hijypuridis (Rostr.) De Wild. 

 In ^Annales de la Societe Bel|je de Microscopie" tome XVII, pag. 46 De 

 Wild em an states that the spores of this species have a ^portion centiale 

 hyaline et refringente, entouree d'une bordure de protoplasme granuleux 

 et plus dense". 



