The lirLLETiN 27 



The bleedings from the tail ami the final lik-cding i)y the tiiroat of a 

 number of hypi'rimmune liogs are tiioroughly mixed, whieh is then tested 

 on suseeptible pigs. The test is made by injeeting two eubie eentimeters 

 of virns into each of four susceptible pigs (25 to 'i') ]>oun(ls) preferably 

 from the same litter. These pigs are then injected witii diiferent amounts 

 of serum. Xo. 1 would get two cubic centimeters of virus and twenty 

 cubic centimeters o{ serum ; Xo. 2, fifteen eui)ic centimeters of serum and 

 two cubic centimeters of virus; Xo. 3, ten cubic centimelers of serum and 

 two cubic centimeters of virus; Xo. 4 would get two cubic centimeters of 

 virus and no serum. If No. 4 dies within fifteen days and Xos. 1, 2, and 

 ;> show no signs of sickness, we then know that the virus used was viru- 

 lent and that the serum protected Xos. 1, 2. and 3 from what would have 

 been a fatal dose of virus. 



Directions for Onleriiiy: Senim 



The serum Avill be shipped, by express, C. 0. D., to any one ordering it, 

 unless check or money order accompanies the order. J )o not fail to give 

 correct address. 



Always state correctly' the amount of serum wanted, or give the weight 

 of each hog to be treated. If a hypodermic syringe is desired, state so in 

 your order, otherwise it will not be sent. A thirty cubic centimeter glass 

 barreled syringe will be sent at cost, if ordered. 



The serum will be shipped in the following size bottles : 



30 c.c, 50 c.c, 150 c.c, 250 c.c, 500 c.c, and 1000 c.c. 



The cost of the senun is three-fourths of a cent per cubic centimeter. 

 NO SERUM WILL BE TAKEX^ BACK; WIIEX THE SERUM IS 

 PLACED m THE EXPRESS OFFICE IT BECOMES YOUR 

 SERUM. 



Address all communications for serum to the State Veterinarian, De- 

 partment of Agriculture, Raleigh, X. C. 



Vaociiiatifui Doses 



It requires more serum per pound of weight to "immunize" young pigs 

 than is required to "immunize" older hogs. 



Cholora-frcc Herds Cholera-infcctcd 



for Scrum Alone Herd for Simulta- 



Melhod ncoua Method 



Suckling pigs 5 to 20 c.c. 10 to 25 c.c. 



25 to 50 pounds 25 c.c. 30 c.c. 



50 to 100 pounds 40 c.c. 50 c.c. 



100 to 150 pounds 50 c.c. 60 c.c. 



150 to 200 pounds 00 c.c. 70 c.c. 



200 to 250 pounds 70 c.c. 80 c.c. 



250 to 300 pounds 80 c.c. 90 c.c. 



300 to 350 pounds 90 c.c. 100 c.c. 



All over 350 pounds 100 c.c. 120 c.c. 



The North Carolina Department of Agriculture assumes no responsi- 

 bilitv. 



