25 



even in the dark-brown peritbecia. One of tLe darkest peritbecia was 

 crushed in water. It contained no asci, but every cell was filled with 

 oily looking- globules which blackened with osmic acid. Here we have 

 the substratum causing change in color, change in form, retardation of 

 the production of asci, and the storage of large quantities of reserve 

 material. Most of the old banana cultures resembled this one rather 

 than the preceding. The dark brown or black color was lodged in the 

 walls of the peridium. The oily granules gave a milky appearance to 

 the crushed contents. In some of these tubes certain microconidia 

 were also observed to have changed into round spores. In all, 17 

 banana cultures were under observation, and for a long time. The one 

 first mentioned seems to have possessed a trace of some substance 

 wanting in the others, and which finally enabled the fungus to fruit 

 normally. 



(4) On Spanish onion steamed in much distilled water the fungus 

 fruited slowly, but finally quite freely, the peritbecia being pale red to 

 bright coral-red. 



(5) On commercial (alkaline) cornstarch steamed in distilled water 

 the fungus grew sparingly and fruited sparingly. At first the peritbe- 

 cia were pale red to coral-red, but after a month they were dark — i. e., 

 almost as black as on the tapioca. Even after 2-^ months there were 

 very few peritbecia. Some were i)ale red, but most of them were dark 

 brown, especially the older ones. There were no asci in these dark- 

 brown peritbecia, but great quantities of oil globules. The mycelium 

 was also modified. The latter was extremely variable in thickness (1 

 to 13 /<), irregular, much inclined to form swollen places and globose 

 ends, or moniliform chains or clumps of big rounded cells. It was also 

 densely packed with refringent globules. In two of the black peritbecia 

 which were crushed in water the peridial wall was brown under the 

 microscoi)e, but normal in structure. 



(C) On crushed wheat steamed in distilled water the fungus fruited 

 quickly and copiously, but the peritbecia were duller red than on potato. 

 In 8 days the surface was densely packed with dull-red peritbecia. At 

 the end of a month some of them which were discharging ascospores 

 were nearly colorless, while others, also discharging ascospores, had a 

 tinge of purple (not red). Other large clumps, which appeared to be 

 full grown, were entirely without color — i. e., a dirty white. Most, bow- 

 evef, were colored on this substratum. At the end of 2^^ months there 

 was a copious protrusion of ascospores. Nearly all of these were glo- 

 bose, brown, with a thick wrinkled epispore, and were 11 to 13 // in 

 diameter. The interior of these spores was guttulate. A very few of 

 the ripe spores were: (a) smooth, (b) broadly elliptical, (c) ovate. Sev- 

 eral hundred were examined in vain for a septum. At this time the 

 walls of many of the peritbecia, protruding spores, were nearly color- 

 less — very pale red or yellowish white. 



(7) On hominy steamed in distilled water the fungus fruited slowly 



