19 



teenth, but it was still mostly white or roseate, there being very little 

 crimson. lu 2, the fungus was white to rose color, with occasional 

 hyphal strands becoming crimson. The color had increased, but it was 

 only about one-half as much as in 1, In 3, the body of the culture was a 

 beautiful crimson mottled with purple. The color was lodged both in the 

 substratum and iu the hyphal strands. Where the rice had shrunken 

 away from the wall a net work of white mycelium was growing out of 

 the crimson mycelium and covering it. In the upper part of the tube, 

 where the fungus projected into the air, it was also white, or rather, so 

 nearly so that it appeared tinted only when taken out and massed 

 on white paper. The conditions in 4 and 5 were like those iu 3, except 

 that there was no i)urple color. In both tubes the rice was shrunken 

 away from the wall and white mycelium was lilling the cavities. This 

 contrasted curiously with the crimson hyphai and rice grains from 

 which it was growing. 



A tube of litmus rice made much more strongly alkaline than the 

 two j)reviously mentioned was inoculated October 28, 1895. ( )n Novem- 

 ber 14 the upper one-third of the rice (that best aerated) was thoroughly 

 invaded by the fungus, the deeper parts being free, or nearly so. The 

 fungus was snowy white. The rice grains invested by the fungus 

 were changed from bright blue to a pale blue, but there was no purple 

 or red color. This culture consisted of 15 c. c. cooked rice, 2 c. c. of violet 

 litmus solution, and three-fourths cubic centimeter of a saturated solu- 

 tion of sodium carbonate. On December 5 the rice had lost all of its 

 bright blue color, but there was no violet or crimson. The entire cylin- 

 der of rice was more or less overgrown and interwoven with the fungus, 

 which was white; the rice grains in the upper part of the tube were 

 greenish gray, and in the lower part a pale purplish rose. 



In cutting through old dense conglomerated rice cylinders overgrown 

 with the crimson fungus, the periphery was generally crimson, while 

 the center of the cylinder was mottled, the exterior of the rice grains 

 being red and their interior white. This seems to be another indica- 

 tion that the pigment requires an abundance of free oxygen for its 

 development. 



Three large Erlenmeyer tiasks of rice were prepared as follows: (1) 

 One-half pound of rice sterilized in several hundred cubic centimeters 

 of distilled water; (2) one-half pound of rice boiled in several hundred 

 cubic centimeters of distilled water, then knocked out of the flask and 

 intimately mixed with one-fourth pound of c. p. calcium carbonate, and 

 subsequently resterilized with more water; (3) one-half pound of rice 

 boiled in several hundred cubic centimeters of distilled water, then 100 

 cc. of a saturated solution of sodium carbonate added, and the sterili- 

 zation completed. These flasks were inoculated at the same time, in 

 the same way, and from the same culture. In 48 hours the condition 

 was as follows : 



In 1, the fungus had begun to make a vigorous growth from four dif- 

 ferent centers of infection; color, i)urplish for the most part, but some 



