18 



purple; in tube 2 (which received twice as much alkali) the fungus had 

 grown through all parts of the rice, and varied from snow white to a 

 faint roseate color, which was scarcely noticeable. In 3 and 4 the 

 starch grains were covered and hidden by the fungus, which was 

 snowy only where it projected into the air above the culture. There 

 were no purple hues, but the body of the culture varied from rose 

 to crimson, the latter color jiredominatiug. There was now most crim- 

 son in the tube which received the most acid, but possibly this was 

 accidental. In 5, all of the blue had disappeared, but the rice grains 

 at the extreme bottom of the tube still showed a very little red j)urple. 

 All the rest of the substratum was so closely invested by the fungus 

 that the rice grains were hidden. The color of the fungus varied from 

 rose color to the most vivid crimson, the latter color predominating, 

 the only white part of the fungus being that which projected into the 

 air at the top of the culture. In 6, which was more alkaline, a half 

 cubic centimeter of rice in the bottom was still violet color, none was 

 blue, and in other respects the culture was like 5. After 40 days 

 1 and 2 still showed the restraining eflect of the soda. In 1, a little 

 of the mycelium was now crimson, but most of it was only pale rose 

 color; in 2, there was much less color than in 1, most of the color 

 being pale rose, but with a little violet and a trace of crimson; the 

 rice had shrunken from one side of this tube and the cavity was filled 

 with white mycelium. In 3 and 4, crimson was still the i)revailing 

 color; both were shrunken away from the wall on one side, and in one 

 tube white mycelium was beginning to fill the cavity. In 6, the lower 

 one- third of the rice was now brilliant crimson, and the upper two-thirds, 

 which had lost much water, was changed to dark purple and partly 

 overgrown with white mycelium. The dark purple color and the crim- 

 son could be seen distinctly in the hyphal strands as well as in the rice 

 grains themselves. In 6, at the end of 58 days, the entire mass was 

 shades of violet, no crimson color remained, and the secondary super- 

 ficial mycelium was white. 



The above experiment was repeated as follows with five test tubes of 

 rice boiled in distilled water : (1 ) Rice, with 3 drops of saturated solution 

 of sodium carbonate; (2) rice, with 6 drops of the soda solution; (3) rice; 

 (4) rice, with 5 drops of one-half per cent hydrochloric acid; (5) rice, with 

 10 drops of one-half per cent hydrochloric acid. All were inoculated 

 at the same time in the same way and from the same culture. In 6 

 days there was a large amount of color in tubes 3, 4, and 5 — rose, purple, 

 and crimson. The color was as well developed in the pure rice as in the 

 tubes which received the acid. Tubes 1 and 2 behaved much as in the 

 preceding series. In 1, at the end of 14 days, the entire rice was 

 invaded, and some of the mycelium was roseate to faint purplish, but 

 there was not one-hundredth part as much color as in the acid tubes. In 

 2, the whole substratum was invaded, and the mycelium was white to 

 faint roseate, there being about one-fourth as much color as in 1. On 

 the twenty-fifth day, in 1, the color was much deeper than on the four- 



