16 



Sugar did not help ou the formatiou of color, but rather seemed to 

 retard it. The litmus reaction was decidedly acid ou the twentieth and 

 twenty-seventh days. 



There was marked retardation of growth in Dunham's solution with 

 2 per cent cane sugar and 1 per cent sodium carbonate. After 20 days 

 the fungus still seemed white, but the fluid was now slightly yellowish. 

 There was less growth than in the acid Dunham. In Dunham's 

 solution containing 2 per cent cane sugar and 0.4 per cent sodium 

 carbonate there was a much better growth on the start than in the 

 same medium with 0.4 i^er cent of malic acid substituted for the soda. 

 At the end of 13 days the fungus filled the alkaline saccharine fluid 

 and was yellowish brown; the fluid was also browned. The brown 

 color in the mycelium was noticed as early as the eighth day. 



The extreme upper part of the gelatin-tube cultures (the substratum) 

 was finally stained rose brown. 



In test-tube cultures 1 to 10 of November 27, 1894, on bread steamed 

 in distilled water (5 consecutive days), the fungus grew luxuriantly. 

 On December 18 the bread was nearly or quite covered and hidden by 

 the fungus. In the top of the cultures where it projected into the air 

 the fungus was snow-white. Farther down this ])ure white color 

 shaded into yellows, purples, flesh tints, and reds, the predominating 

 color being an irregularly distributed bright crimson. All of the tubes 

 showed this crimson color, some more than others; several were very 

 striking. There was less purple and more crimson than in the same 

 fungus when grown on potato. 



In days, on pearl tapioca steamed in distilled water, the fungus 

 was purple. 



In 3 days, on crushed wheat steamed in distilled water, there was a 

 copious growth and a bright purple seam where the fungus rested on 

 the substratum. In 6 days from the inoculation the purple color had 

 extended and a brilliant crimson was visible in the hyphte bordering 

 on that part of the substratum first attacked. The fungus resting on 

 the wheat was purple, while the upper parts of the same mycelium were 

 pure white. 



In 3 days, on hominy steamed in distilled water, the fungus made a- 

 copious growth and developed an abundance of color. This was pur- 

 ple in the greater portion, but crimson in the oldest part. On the sixth 

 day the purple color involved the upper half of the substratum in one 

 tube and nearly the whole of it in the other tube. 



The rice cultures proved particularly interesting. On the third day, 

 in tubes of rice steamed in distilled water, there was a copious devel- 

 opment of mycelium, which was snow-white in the air and i)urplish 

 and crimson on the substratum. In 5 days the bulk of the culture was 

 purplish to carmine. Even the aeriel mycelium was tinged. A little 

 of the mycelium in the bottom of the tube and some on the walls of 

 the tube above the rice was still colorless. After 20 days this crimson 



