LABORATORY EXPERIMENTS. 9 



hdiirs AA'as added to the first and the Avhole shaken out with benzol. 

 This, after takin<r up with acid water, was injected into the same 

 g-uinea pig. The animal soon l)ecame very dull, walkino- with wad- 

 dling oait when forced to move, being evidently alfected by the 

 poison. Two days later the animal was normal. 'J'he urine of a 

 control rabbit, similarlv shaken with benzol, produced no such symp- 

 toms in this guinea pig. 



On February 11, 1907, an aqueous extract of 5 grams was injected 

 subcutaneously into a rabbit weighing ()()().14 grams. An hour and 

 three minutes later the animal was unable to stand, and soon devel- 

 oped convulsive movements of the liml)s. This animal was expected 

 to die at any time during the afternoon, but gradually improved and 

 appeared slightly duller than normal the following day. On Feb- 

 ruary'' 18 it weighed 013. Gl grams; February 25, r)S»,').-20 grams; and 

 on March IG, 552.78 grams, showing a steadv diminution in weight. 



An extract of 0.5 grams injected subcutaneously into a rabbit 

 weighing 2,182.79 grams was without innnediate eft'ect. 



An aqueous extract of 20 grams when injected into a rabl)it weigh- 

 ing 48-1.93 grams soon caused paralysis of the limbs, and the rabbit 

 died in 32 minutes. 



On Felu'uary 11. 1907. a rabl)it weighing 1.417.40 grams was 

 ojDened under ether, and the mouths of the ureters were tied off, 

 together Avith the bladder, to prevent elimination by the urine. An 

 aqueous solution corresponding to 34 grams of the same plant was 

 then fed by mouth. This animal remained dull all the afternoon, 

 and at 4.45 p. m., 4 hours and 45 minutes after the feeding, he was 

 resting with his chin on the cage. He was found dead and stiff at 

 9.15 p. m., having evidently died some time previous. A post-mortem 

 showed the ureters distended and sharply outlined. 



Another rabbit weighing 1,743.37 grams was prei)ared in a similar 

 manner by tying off the ureters and bladder and was fed with an 

 extract of 50 grams. In 1 hour and 47 minutes this animal was 

 paralyzed and unable to stand, breathing very slowly, and died 6 

 minutes later. The ureters were found dilated as in the previous 

 case, and the stomach was slightly reddened. 



A control for these two rabbits was prepared in a similar manner 

 at the same time as the others. Xine hours after the operation, when 

 the two rabbits which were fed the Delphinium were dead, this rab- 

 bit was bright and running about, but was then killed with chloro- 

 form to prevent suffering. 



In these cases it is necessary to consider not only the effects of non- 

 elimination but also the fact that the power which the liver possesses 

 of storing up and removing injurious bodies from the circulation may 

 iii-i 



