27 



Feb. 6th and 7th.— Left nostril of hen No. 10 shows a small quantity of 

 serous, odorless fluid, which had disappeared the next day. These two hens 

 remained normal and healthy 



Fowls No. 6. a., b., c, d., and e., were kept with the diseased chickens 

 during the month of December. 1901. and January, 1902, without becoming in- 

 fected. Later these chickens were used for experimenting with the roup 

 bacillus, which had been made- virulent by passage through pigeons. All re- 

 acted with typical symptoms of chicken diphtheria. 



Experiments to Show if Healthy Bikds Could be Infected wtih Secretion 



OR Exudations of Diseased Ones. 



Fowl No. 4. Young, healthy hen. 17th Dec. 1901. Some of the solid, 

 cheesy peritoneal exudation from diseased fowl No. 4. was rubbed on the 

 sound or slightly scratched membranes (mucous) of both eyes, the mouth, 

 and in the 'higher parts of the nostrils. 



Jan. 18.— General condition normal. At the small scratch wound in the 

 mout/i the membrane is slightly swollen and red. A little clear, serous fluid 

 f^xudes from the right nostril. 



Jan. 19.— nMouth normal. Both nostrils are moist, with a little serous 

 liquid having no smell. 



Jan. 21.— The secretion from the nostril is grey, slimy and has a putrid 

 smell. On the mucous membrane of the right side of the mouth, an irregular 

 yellowish-white spot is located. It is about i m.m. thick and 1-2 cm. in size. 

 This patch was taken of¥. and the membrane under it bled at a few spots. 



Jan. 14.— Chronic, putrid nasal catarrh. General condition unchanged. 

 Mucous membrane in the mouth always very pale, sometimes with small 

 pseudo-membranes. 



Jan. 21.— In the middle of the palate, a pseudo-membrane is located, about 

 J4cm.2 in size, and i to i^'< mm. thick. It was removed and rubbed on the 



left conjunctiva. 



Jan. 22. — The membrane has reformed in the mouth. Eyes are normal. 



Jan 2i. — Some new membranes have formed at different places in the 

 mouth. 



From the 27th of Jan. till the middle of ]\Iarch, the chronic nasal catarrh 

 disappeared, after having ceased several times for only one or a few days. 

 The local infection in the mouth disappeared at the same time. General con- 

 dition was good. 



Fowd No. 5a. Healthy hen. half year old. On the 17th of December, some 

 of the putrid nasal secretion from diseased chicken No. 4 was rubbed in and 

 upon the mucous of the eye and the regio olfactoria. 



This hen never became sick or diseased. 



Fowl No. 7. .\ hen like No. 5a was inoculated on Jan. 3rd. 1902. with 

 nasal secretion and pseudo-membranes from fowl 5. Inoculation on and 

 under the mucous membrane of the mouth, and on the mucosa of the regio 

 olfact. No visible reaction followed. 



