28 



Fowl No. 9. This fowl suffered from chronic diarrhoea. It was kept in 

 the stable for four months and then inoculated in eye-lids, mouth, and nos- 

 trils with croupous material from the eye-lid of diseased chicken No. il. 



Fowl No. 8. Healthy cock, three years old. Had been kept in the stable 

 continually for three months. 



Jan. 4. — On the scratched mucous membrane in the mouth near the tongue, 

 a croupous membrane from fowl 3 was rubbed in. 



Jan. 5.— The mucous membrane at the place oi infection was red, and 

 covered with much saliva. General condition, normal. 



Jan. 6. — At the place of infection, the mucous membrane is uneven grey ; 

 at several places, yellowish-white points appear. 



Jan. 7. — Near the tongue, a croupous membrane has formed, yellowish- 

 white in color, i mm. thick and about i cm. in area. The surrounding mucosa 

 is reddened. After having taken off the membrane, the mucosa begins to 

 bleed. At several other places in the mouth, very thing, greyish pseudo- 

 membranes formed. 



Jan. 8. — The pseudo-membrane near the tongue, which had been removed 

 yesterday, has reformed, and is larger than before. 



Jan. 15.- — At the primary seat of infection, the croupous membranes have 

 disappeared, but a small croupous patch is located on the left side of the 

 mouth, and there are four others upon the mucous membrane of the palate, 

 near the entrance to the nasal cavity. 



From the i6th Jan. till the 25th Feb. — 0;ten pseudo-membranes appeared 

 on the mucous membranes in the mouth, and disappeared again in a few days. 

 The general condition at this stage was poor. All visible mucous membranes 

 were pale. 



This fowl was killed and the post-mortem examination showed the exist- 

 ence of anaemia. No growths in cultures from the spleen. 



Fowl No. 21. Young, healthy hen. 



Jan. 3. — Was inoculated with slimy, putrid, nasal secretion from fowl 

 No. I, containing very many spirilla. i^ c.c. was injected into the mucosa 

 and submucosa of the mouth and nose, Yz c.c. into the right eye-lid and V2 c.c. 

 into the right pleural cavity. 



Jan. 4. — General condition unchanged. Eye-lid slightly swollen and con- 

 junctiva moist. On the palate, one pseudo^membranous patch has formed, 

 % cm. in size, yellowish-white and cheesy. Mucous membrane of the mouth 

 is pale. 



Jan. 7, and later. — General and local condition normal. 



Fowl No. 24a. — This bird was inoculated the 23rd of January, 1902. with 

 material taken from the cheesy, degenerate lymph follicles in the oesophagus of 

 fowl No. I. 



Fowl No. 25. — This chicken was inoculated with a solid mass of yellow- 

 ish pus taken from the eye-lid of diseased fowl No. 21. 



In both cases, the eye-lids became swollen for a few days. The mouth 

 remained unchanged, and the general condition was never disturbed. 



Besides these experiments, we tried to infect six other chickens (6-12 



