30 



Fowl No. 4. Weak hen, about ^ year old. 



Jan. \2. — Inoculated in the mouth with an anaerobic bouillon culture, 48 

 hours old, made with the same material from diseased chicken No. 9. It 

 contained coli and roup bacilli, and a streptococcus. 



Jan. 13. — At the place of infection in the mouth, two small white spots 

 have formed. 



From 14th of Jan. till loth of Feb. — General condition, normal. Several 

 times, small croupous spots formed on the mucous membrane in the mouth 

 for a few days. 



Fowl No. 18. Healthy young hen. 



Jan. 12. — Was inoculated with an aerobic culture from diseased hen No. 

 9 in the mouth and under the right conjunctiva. This culture contained the 

 roup bacillus, and some long and thick rods, and a few cocci. 



Jan. 14. — The infected eye-lid is slightly swollen. Mouth, normal. 



Jan. 15th till Jan. 23rd. — General condition always normal. Swelling- 

 of the eye-lid disappeared. Mouth never affected. 



Fowls 22 and 2^. \bout 6-month-old hens. 



Jan. 21.- — A young agar culture of the roup bacillus, isolated from fowl 

 3, was inoculated in the mucous membranes of the mouth and nose without 

 any abnormal changes subsequently taking place. 



Fowl 26. Healthy hen, i year old. 



Had suffered from natural roup at the beginning of winter, but wholly 

 recovered after being diseased for two months. 



March 15. — % c.c. of a young culture of the roup bacillus, isolated fr(nn 

 an infected pigeon, was inoculated in the left superior eye-lid, and in the 

 pleural cavity. 



March 16. — ^General condition very much disturbed. Eyes were kept 

 shut, left eye-lid swollen, and warm. The conjunctiva is very watery, and 

 grey. Under the eye-lid, there is much sHmy matter. The bird breathed 

 through the beak with loud inspiratory noise. 



March 17. — General condition much improved. Respiration through the 

 beak, swelling in the eye-lid diminished. Killed by decapitation. 



Post-Mortem : Carcass in a fairly well nourished condition. Conjunctiva, 

 pale and watery. In the depth of the submucous tissue of the left eye-lid, a 

 solid mass of grey pus, about i]/- cm. long, is found. It can be extracted with- 

 out breaking, being surrounded by a smooth brown membrane. At several 

 places in the periorbital tissue, similar pus masses are present, but was sharply 

 separated from the surrounding tissue. The interior eye is in tact. The nasal 

 mucosa in the regio olfact. is highly reddened and covered with much slimy 

 putrid secretion. In the mucous of the left lacrymal canal are small bloody 

 extravasations. Lungs are oedematous. 



Fowl No. 27. Small healthy hen, about one year old. 



Had several times been inoculated with the roup bacillus without result. 

 On the 28tih of April, this bird was again inoculated with a young agar culture 

 of the roup bacillus, which had been passed through two pigeons. ^ c.c. was 

 injected into each of the upper eye-lids, and 3 c.c. in the right pleural c?vity.. 



