32 



Fowl No. 30. Healthy hen, about 2 years old, had been diseased with 

 roup at the beginning of the winter ; but apparently healthy when inoculated. 

 March 17.— Was inoculated with the solid matter (suspended in sterile 

 water; irom the eye-lid of fowl No. 26. A few drops were injected into the 

 right upper eye-lid, i c.c. in the right thoracic cavity. The solid matter con- 

 tained leucocytes and roup germs. 



March 18.— Small swelling of the eyelid, without pathological scretion of 

 the mucous membrane. General condition, very much disturbed. 



March 20. — General condition, better. Sometimes the hen coughs. Swell- 

 ing in thf eye-lid mostly disappeared. 

 Later, the fowl recovered entirely. 



Fowl No. 31. Had been diseased before. Inoculated in the same man- 

 ner as fowl 30. After 24 hours, the general condition was very much dis- 

 turbed, the eye-lid swollen, but the bird recovered in a few days. 



Hen No. 32. Hen, gray, about one and a half years old. Had suffered 

 with natural roup till New Year. Healthy now. 



March 19. — Inoculated with a small quantity of the cheese-like matter from 

 pigeon 12 (which had been inoculated with the roup bacillus) on the mucous 

 membrane of the mouth and in the pleural cavity. 



March 20.— General condition disturbed. A croupous membrane has form- 

 ed in the mouth, about 54 cm. in size and i mm. thick. It can easily be re- 

 moved. The mucous membrane under it is uneven and grey. 



March 2b. — General condition, better. At several places in the mouth cav- 

 ity, small pseudo-membranes have formed. Appetite lacking. 



April 8. — Great loss of flesh. General condition, very bad. Fowl general- 

 ly lies on the 'ground and does not care for food. Killed. 



Post-Mortem: On the left side is a chronic indurative pleuritis (pleura thick 

 and uneven, gray); liver small, spleen very large, without germs. The interior 

 of the thin boweU contain a little food mixed with much slime. In the mucous 

 membrane are petechial spots at many places. 



Fowl No. 33. — Small hen, abont 2 years old, had been diseased with roup. 

 Was inoculated like fowl 32. Only a quirkly passing disturbance of the general 

 condition was tihe result. 



Fowl No. 34. — Large, healthy hen that had never been diseased, about i;-2 

 years old. 



March 24.— Inoculated with agar culture (28 hours old) isolated from pigeon 

 20 (second passage through pigeons). The culture was rubbed in the mucous 

 membrane of the mouth, and 1-8 c.c. was injected under the mucous membrane 

 of the upper eye-lids. 



March 25. — General condition disturbed. Hen takes no interest in its 

 surroundings, but cowers in a corner of the cage, with the neck drawn close to 

 body, and eyes shut. The right, upper eye-lid is considerably swollen, and has 

 its skin reddened. Conjunctiva gray. The left upper eye-lid is slightly swollen. 

 Mucous membrane in the mouth is pale. Several yellowish-white pseudo-mem- 

 branes are located on the tongue, and tlie mucous memlM-ane near it. The 

 submucous tissue on the bottom of the mouth cavity is oedematous. 



