36 



April 4. — The eye-lids were inflamed, hot and sticking together. Under 

 the eye-Hd, there is much slimy lacrymal secretion, and a jelly-like mass. The 

 places of inoculation in the mouth are covered with thin pseudo-membranes. 

 General condition disturbed. Later on, several small croupous membranes 

 formed on the mucous membrane in the mouth. 



April II. — The whole eye-lid was filled with a cheesy, solid mass, which 

 could easily be removed. Conjunctiva dirty gray. On the upper eye-lid a 

 fairly large pseudo-membrane has formed. The next day the cheesy mass 

 had reformed larger than before. 



April 24. — A large solid tumor has formed in the depth of the upper eye- 

 lid. The pseudo-membrane could scarcely be removed. Later, the putrid 

 conjunctival catarrh ceased, the pseudo-membrane disappeared, the tumor in 

 the eye-lid remained unchanged. The general condition was slightly disturb- 

 ed, and the hen lost flesh. 



Fowl No. 39. Inoculated with a Roup culture, which had been passed 

 twice through pigeons, but then cultivated on agar for three weeks. The eye- 

 lids became swollen, but the swelling soon subsided. General condition re- 

 mained normal. The culture, therefore, had lost most of its virulence. 



Fowls No. 42-45. Healthy young bens, inoculated with freshly isolated, 

 virulent Roup bacillus, on the mucous membrane in the mouth. Everywhere 

 croupous membranes appeared at the places of inoculation. There they re- 

 mained for a few days, and generally reappeared after removing once or twice. 



The most severe reaction occurred in fowl 43 the day following the in- 

 fection ; the general condition was very much disturbed, the beak was kept open, 

 and much saliva was found in the mouth. The palate was covered with a 

 whitish croupous membrane. Other membranes were located in the pharynx. 



The last pseudo-membranes disappeared in all these infected fowls in two 

 or three weeks. 



Pigeon Inoculations. 



Pigeon No. i. This bird was inoculated in both eye-lids, and on the 

 mucous membrane in the mouth, with cheesy matter taken from the wattles 

 of diseased fowl No. 24. 



Feb. 18, 1902. — The only result was a swelling of bhe eye-lids, which quick- 

 ly disappeared. 



Feb. 27. — Condition normal. . Some croupous membrane taken from a 

 diseased fowl was rubbed on the mucous membrane of the pigeon's mouth, and 

 on the conjunctiva. 



March 4. — Normal. Inoculated % c.c. of a culture of the roup bacillus, 

 which had been grown for several months on agar. The next day the bird 

 was weak and somnolent, with no appetite. Both eyes are kept shut, the eye- 

 lids sticking together ; conjunctivitis. 



April 17. — The acute conjunctivitis generally became chronic and putrid, 

 but disappeared after a while. No pseudo-membranes formed on the con- 

 junctiva, but several of them appeared on the mucous membrane of the mouth 

 for several times. The general condition was better. 



