37 



Inoculated 2 drops of a roup culture (24 hours old) in the left conjunctiva 

 on the upper eye-lid, and into the mucous membrane of the mouth. 



April 18. — General condition very much disturbed ; eye-lids sticking to- 

 gether, and swollen. The lid-sack contains slimy tears. A whitish pseudo- 

 membrane has formed on the palate. Inoculated 3 c.c. of a filtered (sterile) 

 bouillon culture under the skin. 



April 19. — Condition like yesterday. The filtered culture has been entire- 

 ly absorbed. 



April _-8. — The chronic catarrh of the conjunctiva still exists. In the depth 

 of the upper eye-lid, a solid tumor has formed. Good general condition. 



Pigeon No. 4, This bird was inoculated on the 18th of February with 

 cheesy masses, taken from the lungs of fowl 41 (B. pyocyaneus), on and in the 

 mucous membrane of the eye and mouth. 



Feb. 25. — General condition, not disturbed. The day following the in- 

 fection, small croupous membranes formed at several places in the mouth. 

 The whole palate is covered with a yellowish-white diphtheritic membrane 

 which was difficult to remove ; the mucous membrane was uneven, gray and 

 bleeds. The eye-lids are slightly swollen. 



March 6. — The pigeon is apparently normal. V^ c.c. pure culture of the 

 roup bacillus was inoculated into the left pleural cavity. 



^larch 8. — 'General condition very badly disturbed. 



March 13. — General condition has slowly improved. All mucous mem- 

 branes are very pale. On the palate, a small croupous membrane has formed. 

 Killed. 



Post-Mortem: Left lung is firmly adherent to the pleura. The central 

 part of the lung, that is, the region of the branching of¥ of the larger bronchial 

 tubes from the wind-pipe are solid, and inflamed. In the centre 01 this in- 

 flamed area is a solid, dry cheese-like, yellowish-white mass, measuring about 

 I c.c. in diameter. This mass is partly surrounded with a smooth red-brown 

 membrane. Spleen is small, and cultures made from it remained sterile. 



Pigeon Xo. 3. Inoculated the i8th of February in the same manner as 

 pigeon 4, and in addition i c.c. of the cheesy mass, triturated in sterile water, 

 was injected in the left pleural cavity. The general condition was very much 

 disturbed for the first 3 days after inoculation. The eye-lids were swollen, and 

 a few small croupous membranes appeared in the mouth. 



Feb. 27. — Condition normal. Some of the mucous membrane from fowl 

 No. 27 was inoculated, after trituration in sterile water, into the mucous mem- 

 brane of the eyes and mouth. No reaction followed. 



March 6. — 1-5 c.c. of a culture of the roup bacillus was injected into the 

 left upper eye-lid. The eye-lid became very much swollen, and an acute ser- 

 ous conjunctivitis developed, and became slimy and putrid. h solid tumor 

 formed in the swollen eye-lid. The general condition was much disturbed at 

 the beginning and slowly became better. 



May 6. — General condition normal. Local conditions have disappeared 

 with the exception of a solid tumor in the upper eye-lid. 



Pigeons No. 5. 6. 7. 10. and 11. These birds were inoculated in the mouth 



