42 



infected birds did not show any apparent influence on the roup bacillus. In 

 test tubes, traces of an agglutination appeared in proportions of 1:50 or 1:100. 

 The bacillus does not form a strong toxin. Old cultures filtered through por- 

 celain inoculated in doses of 3-6 c.c. produced no local or general disturbances. 



Bouillon cultures heated to 65 degrees c. for 10 minutes and inoculated in 

 doses of 1-4 to I c.c. into the eye-lids of healthy fowls produced considerable 

 inflammation, which disappeared in 2 days. 2-5 c.c. inoculated into the pleural 

 or peritoneal cavity disturbed the general condition for i to 3 days, and pro- 

 duced trembling of the muscles. 



We attempted to immunise several fowls and rabbits with the roup bacillus. 

 first injecting small quantities of heat killed cultures, followed by small doses 

 of living cultures. The amount inoculated was slowly increased, but as soon 

 as we liegan to use large amounts, small tumors formed beneath the skin. These 

 became solid, hard, and had no tendency to become absorbed or to produce 

 abscesses. Tlu-y remained under the skin and led to chronic anaemia and death 

 ni th"^ animal. After intraperitoneal injection of the cultures, the animals be- 

 came chronically diseased, and grew thin and anaemic. The rabbits very often 

 suffered with a secondary, putrid inflammation of the eyes. 



These attempts to produce immunity were finally abandoned, as it seemed 

 impossible to produce immunity 'by the methods described above. 



Experiments to Infect Healthy Fowls with Bacilous Pyocyakeous. 



Fowl No. 41. Healthy hen, about i year old. 



Jan. 12. — Was inoculated with a 24-hour-old culture of B. pyocyaneus which 

 had been isolated from diseased chicken No. 19, by rubbing a little of the cul- 

 ture upon the mucous membrane of the eyes, and injection of ij^ c.c. into the 

 pleural cavity. 



Jan. 13. — General condition considerably disturbed. There is hot swell- 

 ing of the left eye-lids, serous conjunctivitis. Conjunctiva is gray, swollen, 

 and at several places covered with croupous membranes, which are easily re- 

 moved. 



Jan. 26. — General condition more or less disturbed ; no appetite ; putrid 

 conjunctivitis of the left eye ; the cornea is turbid, gray, and uneven ; the beak 

 is generally kept open for breathing. ]\Iucous membrane is gray and covered 

 with much slimy saliva. 



p^.l) i7_ — 'General condition is very much disturbed. The fowl is almost 

 a skeleton, and lies in a corner of its cage in a somnolent condition. The re- 

 spiration, especially the inspiration is very irregular. Mucous membrane and 

 crest are cyanotic. A chronic, putrid conjunctivitis exists on the left side. 

 Cornea gray: right eye seems to be normal, but the fowl is unable to see. 

 Killed. 



Post-^liirtem : Left cornea is uneven, gray, and covered with a thm. 

 putrid mass. The corpus vitreum is turbid, partly liqucified, and mixed witli 

 parts of the destroyed retina. A solid mass of cheesy matter has formed in 

 the pleural membrane between heart and lungs, and the surrounding membrane 



