43 



i. changed into a thick mass (2-3 mm.) of granulation tissue of a brown color. 

 On the left side, all bronchial tubes in the region of the larger branches ot the 

 wind-pipe are fiUed with solid cheesy matter. The lungs themselves contam 

 a few small pneumonic spots, which contain cheesy centres. The B. Pyocyaneus 

 was isolated from all diseased parts. The cheesy mass itself contains nuclei 

 of epithelial cells and a few real protozoa, motile, with short cilia (see Fig. 21.)^. 



Fowl No. 41a. Was inoculated on the mucous membrane of the mouth 

 and nose with the same culture as fowl 41- The next day (February I3th\ 

 the mucous membrane in the mouth was reddened, and showed a few blood- 

 extravasations. These symptoms soon disappeared. 



Fowl No. 46. Healthy, gray hen. 



April 18.— Was inoculated with 1-5 c.c. of a young culture of B. pyocyaneus 

 under the conjunctiva of the left upper eye-lid. and on the scratched mucous 

 membrane of the mouth. 



April 19.— General condition unchanged. Eye-lids hot, swollen, and stick- 

 ing together. Conjunctival sack contains a slimy, putrid secretion. :\Iucous 

 membrane in the mouth is gray and covered with slimy saliva. 



April 21.— On the conjunctiva of the upper eye-lid. a pseudo-membrane has 

 formed. It was removed, and the mucous membrane began to bleed. Several 

 other membranes have formed on the mucous membrane near the the larynx 

 (1-2 mm. thick). 



April 25.— The condition is about the same. Putrid conjunctivitis and ap- 

 pearance of pseudo-membranes. The membranes around the larynx reappear 

 after removal. A putrid secretion from the right nostril has appeared. The 

 general condition of the fowl is much disturbed, but appetite still exists. 



May 2. — Dead. 



Post-^Mortem : Thin carcass. Mucous membrane in the mouth pale. Be- 

 tween tongue and larynx a cheesy croupous membrane has formed, which is 

 easily removed. On the edge of the larynx, a few thick pseudo-membranes 

 have formed. The laryngeal cavity is almost filled up with pseudo-membrane- 

 ous, cheese-like masses. The membranes are either located on the mucous 

 membrane, or directly on the cartilage. The mucous membrane is entirely 

 transformed into croupous membranes (see Fig. 10). The upper eye-lid con- 

 tains a solid cheesy mass, which was attached to the surrrounding pyogenous 

 membrane by a few thin fibres. The conjunctiva of the same eye-lid is partly 

 covered with a pseudo-membrane. The mucous membrane of the nostril is soft 

 and covered with a putrid, slimy mass containing much solid cheesy matter. 

 The nasal secretion has an offensive smell. 



Fowl No. 47. Was inoculated on the i8th of April in the same manner 

 as fowl No. 46. A putrid conjunctivitis formed. Diphtheritic membranes 

 appeared in the mouth. The general condition was much disturbed at the 

 beginning, but improved after a while. The fowl, however, grew thinner, be- 

 came badly diseased again, and died the 25th of June. (See Fig. 7.) 



Post-Mortem : The eye-lids were swollen, and the whole orbital cavity was 

 filled up with a solid cheesy mass and offensive, stinking matter, the eye 

 having disappeared. 



