Positive results were obtained in each case, with the characteristic 

 symptoms of the disease, viz., rotting and blackening of the leaves 

 and stem. 



These plants were all kept under favorable conditions for the 

 spread of the rot. These conditions are described at length later on 

 in this paper. 



4. The diseased, or rotted, tissues contained the Bacillus oleraceae in 



huge numbers. 



While their distribution and effect on the tissues was the same 

 as that met with under ordinary field conditions, and re-isolation 

 proved beyond doubt that it was identical with the organism which 

 was inoculated. 



5. The chemical prodALcts of tJie organism also produced the charac- 



teristic symptoms of the disease. 



The bacillus was grown on raw turnips and cabbage until all the 

 tissues were completely rotted, and the rotted material was then 

 pressed and the juice extracted and forced through a Chamberland 

 filter. This filtrate, which was found to be sterile, produced soften- 

 ing and rotting when placed on cut surfaces of raw potato, turnip, 

 caulitlower and cabbage. Control cultures of these vegetables, kept 

 under the same conditions as the inoculated slices, remained sterile. 



Pathological Histology. 



A microscopical examination of the soft pulp from cauliflowers 

 and turnips showed the presence of enormous numbers of bacteria. 

 Xo mycelium or fungus spores were present. The bacteria, were 

 actively motile. In fre.sh preparations, free plant cells were visible 

 and many were much disorganized. 



A large variety of diseased tissues were fixed in a saturated 

 solution of corrosive sublimate in 94 per cent alcohol, and subse([uently 

 imbedded in paraffin. Some 400 sections were cut from various 



