•more rapidly, and the marked swelling which we see in the cauli- 

 flower is either absent or less well developed. 



Fig. 11 shows the bacteria in the intercellular spaces, the slight 

 swelling of the cell walls and the beo-inninsf of the disororanization 

 of the cells. 



Inoculation Experiments. 



It was found impossible to perform trustworthy inoculation 

 experiments with plants grown in the field, as in this locality the 

 white turnips and cauliflowers were more or less affected with the 

 rot, hence it was necessary to grow fresh plants, from clean seed, in 

 soil which had never been used for growing these plants. On account 

 of the lateness of the season, the plants were grown under glass and 

 all the following experiments, unless otherwise stated were conducted 

 on pot-grown plants in a greenhouse with an average day temperature 

 of 20-25' C. 



Series 1. 



Three plants each, of cabbage, cauliflower, rape, white turnip, 

 swede turnip and kale were inoculated with needle punctures through 

 the parenchyma of the leaves. The platinum needle was dipped into 

 a twenty-four hour bouillon culture of the organism, and from three to 

 Ave punctures were made on one or two leaves of each plant. 

 Results : — 



Cabbage. In two days, the inoculated leaves were flaccid and 

 whiiish brown in the vicinity of the punctures. This area increased 

 slowly for five days and then dried out. 



Cauliflotver. In two day, there was a flaccid, papery area 

 surrounding the punctures ; in five days, all leaves were rotted and 

 had dropped down parallel with the stem of the plant. 



There was no subsequent infection of the stems ; the leaves 

 gradually dried off at the base of their petioles. 



Baj)e. No results followed inoculation. 



White Turnip. Slight infection was produced around the punc- 

 tures ; but the lack of moisture seemed to hinder further growth. 



Swede Turnip. No results followed the inoculation. 



Kale. No results followed the inoculation. 



