6 



Control Plants of each species, pricked with a sterilized needle- 

 remained perfectly healthy. 



This series, therefore, showed that the inoculation of the germ 

 made with needle pricks in the parenchyma of the leaves, pro I need, 

 more or less disease in cauliflower, cabbage and white turnips. The 

 absence of sufficient moisture in the greenhouse, however, prevented 

 the disease from becoming thoroughly established. 



Series, II. 



This series was a duplicate of Series 1, only an agar cultuie of 

 the organism was used instead of a bouillon culture. 



The results were similar to those in series 1, with the exception 

 of the Swedes, which became slightly infected. In one plant, a whole 

 leaf rotted and fell oti' the plant; but the petiole subsequentl}' dried off. 



Series III. 



In this series, three plants each of cabbage, cauliflower, rape, 

 wdiite turnip, Swede turnip and kale, were inoculated with needle 

 punctures through the veins of the leaves. The needle was dipped in 

 a 24 hour old bouillon culture, and from 3 to 7 punctures were made 

 on one or two leaves of each plant. 



Control plants were punctured in the same manner ; but with a 

 sterilized needle. 



Results : — 



Cabbage. In two plants there was no apparent change; in the 

 other plant a small cavity lo m.m. long and 5 m.m. wide had been 

 formed on the mid-rib by the rotting of the cells ; but this subse- 

 quently dried out and the leaf remained healthy. 



Cauliflotver. No results followed the punctures of the veins and 

 mid-rib. 



Rape. In ten days, the leaves of all three plants were slightly 

 affected, the vein was split, and a watery exudation was present on 

 the surface. The inoculated leaves began to droop ; but the disease 

 progressed no further, and the wound became callused and partly 

 healed. 



White Turnijj. The inoculated leaves behaved in exactly the 

 same manner as the inoculated rape leaves. 



Swede Turnips. No results followed the punctures of veins and 

 mid-rib. 



