Kale. No results followed the punctures of veins and mid-rib. 



This series, as a whole, gave less harmful results than the inocu- 

 lation of the parenchyma. In cabbage, rape, and white turnip some 

 slight disease symptoms were produced ; but there was no general 

 infection of the plant. Lack of moisture seemed again to prevent the 

 rapid development of the disease and perhaps the different composi- 

 tion of the vascular cells hindered the formation of cell-destroying 

 enzvmes. 



Series IV. 



In this series, three plants of each of the five species already 

 mentioned were used. A small portion of the epidermis on the upper 

 part of the base of a leaf-stalk was removed and two loopfuLs of a 

 bouillon culture were rubbed on the exposed portion. 



Results : — 



Cabbage. The leaf-stalk rotted through in three days and the 

 leaf fell off" from its own weight. The rotting did not effect the stem, 

 as the diseased tissue dried out. 



Caulijiower. There was slight rotting, or softening, in two days, 

 and in five days the leaf rotted off", and the portion next to the stem 

 dried up. 



Rape. Slight rotting occurred for three days, when the wound 

 dried up. 



White Turnip. In two days, the softening of the tissues at 

 point of inoculation had extended across the petiole. In five days, 

 the leaf fell off, the rotting extending all through the stalk. The in- 

 fected base then dried and healed. 



Sicede Turnip. Behaved the same way as the white variety. 



Rale. In three days, the leaves were so much rotted through 

 that its own weight caused it to break off". The wound then dried up. 



Control Plants with the epidermis removed, but with no inocu- 

 lation, remained healthy. 



In the above account of this series, the results are given for only 

 one plant of each species, the two remaining plants of each lot behaved 

 in a similar manner. These plants showed considerably more disease 

 than those inoculated by vein or parenchyma punctures. This was 

 probably owing to more moisture being present. At the juncture of 

 the stalk with the stem, small drops of water would collect from the 

 leaf surface, thus providing more moisture for the bacteria. As soon 



