8 



as the leaf had rotted off, or fallen by its own weight, no more water 

 collected in the angle of leaf and stem and the tissues rapidly dried 

 up. 



Series V. 



In this series one plant of each of the species alread} mentioned, 

 was used. 



The lowest leaf of each plant was cut off, about an inch from its 

 juncture with the stem. The cut surface was then rubbed over with 

 a platinum loop, charged from a bouillon culture of the organism. 

 Check plants received the same treatment, without inoculation. The 

 results were very similar to Series IV. and need not be repeated in 

 detail, Rotting usually extended downwards toward the stem for 

 about half an inch, or even as far as the juncture with the stem, and 

 then dried ont. The check plants showed no signs of rotting. 



Series VI. 



Three cauliflower plants were inoculated at the base of the petiole 

 with a boudlon culture by means of two or thrte needle pricks. In 

 two days, there was rotting, the affected area being 3x7 ni.m. One 

 of these plants was then placed under a bell-jar and at the end of six 

 days the incculated petiole and leaf were completely rotted, the leaf 

 fell oft" in a mushy mass and the rot spread to the stem, infecting 

 the whole plant. 



The flower head, which was well developed and quite white, 

 gradually changed to a brown color and then rotted. The plant was 

 practically destroyed 14 days from infection. 



The diseased area in the other two plants (kept in the same state 

 except that no bell-jar covered them) gradually dried out, leaving a 

 small hole caused by the rotting of the tissues. 



Subsequently, this experiment was repeated several times with 

 the same results, the plant under the l)ell-jar rotting leaf by leaf, with 

 final rotting of the flower. 



Fig. 2 shows the beginning of the rot, a leaf (the one inoculated) 

 having rotted thi'ough at the base of the petiole and fallen off The 

 stem of the plant, just below the crown, was darkened, due to the 

 softenins: and discoloration of the tissues and the lower leaves are 

 beginning to wilt, owing to the cutting off of their supply of nourish- 

 ment. The leaves of the healthy plant, as shown in Fig. 1, are erect 

 and rigid and comparison with tlie inoculated plant in Fig. 2 shows 



