16 



always most nunu-rous on the outside of the cheese-like matter in nostrils, 

 eyes, and bronchial tubes, as well as on the underside of the pseudo-membrane, 

 or in the material which was scraped from the mucous membrane. The other 

 protozoa-like bodies were present in the greatest numbers in material from the 

 same source. If we compare the drawings of the different bodies in (Figs. 14- 

 25), we find very many forms which agree with the protozoa found by Rivolta, 

 Silvestrini, Magganti. Piana, Pfeififer, Babes, Puscariu, and Galli-\^alerio. 





i>-0:5? 



Fig. 14. — Protozoa-like bodies from the lironcliial exudate of Fig. 15. — Bodies from tlie slimy purulent 



pigeon No. 1. a The nuclei of leucocyte.'-. b. Swollen nasal secretion of pigeon No. 12. Same 



epithelial nuclei with or without nucleoli. /. Fat drop- lettering as figure 14. 

 lets. /(. Yeast cell with spores, z. Probably a giant cell. 

 Epithelial nuclei lia\ e a diameter of s-io u, the othei- 

 bodies are drawn on the same si-ale. 



Round, amoeba and crescent-shaped forms, etc., were often present and the 

 contents of these bodies were homogeneous, granular, or partly homogeneous 

 and partly granular. They stained either badly, or not at all. Very often, one 

 or more nucleoli were present which appeared in unstained preparations as 

 clear or reddish spots. In stained preparations, the one small nucleolus was 

 often stained, while the other in the same cell had not taken the stain at all. 

 Besides these central bodies, the protozoa-like forms often held enclosed larger, 

 clear vacuoles, which could be stained black with osmic acid, and were, there- 

 fore, fat globules. Other similar globules as they appeared in pigeon 4, and 

 hen 7 gave no fat reaction and might have been true protozoa, but were more 

 probably yeast-cells. Such accidental existence of foreign bodies in the exuda- 

 tions is not a rare thing, as is shown by Figs. 18, 19, 22 and 23. Very many 

 of the protozoa-like bodies were either enclosed in the remains of cells, or 

 showed debris of cells that had been present. 



Twice, motile protozoa were present. In the bronchial tubes of infected 

 hen No. 41. there were some oval forms which moved by means of a wreath 

 of short cilia around the head end. (Infusorium diplodinum (See Fig. 22, i). 



On the surface of the firm exudations in the mouth of pigeon 6, round, 

 granular forms, each with three long cilia, were present. There is nothing 

 remarkable in this, for motile protozoa were frequently found in the mouth 

 cavity and intestines of perfectly healthy fowl. 



