lb 



uas suinelmifi i^ulaicu, ana a large number of other bacieria-cocci, bacilli and 

 a spirillum. ihe spirillum which had three or four turns could not be grown 

 in any culture medium. Some five or six of the other micro-organisms were 

 grown in pure-cultures, and their pathogenic properties were investigated by 

 inoculations into guinea pigs, mice, and chickens. No results followed these 

 inoculations ; and, consequently, we must regard these forms as purely ad- 

 ventive. 



Fig. 18. —From the cheese-like exudate from the 

 cella infiaorbitalis of pigeon 6. rf . Is one of 

 the Flafrellata with three motile fla>rella. 



Fig. 19. — From the underside of a diphtheri-mein- 

 hrane from hen Xo. 7. p. Probably some form 

 of veg-etable life. 



We succeeded, however, in isolating two virulent forms of bacteria. The 

 one was a typical Bacillus pyoc3'ianeus. This bacillus was present in the cheese- 

 like masses in the lungs of hen 9, in company with a few B. coli ; in the firm exu- 

 dations of the Cella infra-orbitalis of hen t,2 ; in the cheesy masses of hens 29, 

 30, 31, 33, 34, 35. 36. In the six cases last mentioned, the bacillus was also 

 isolated from the blood, liver, kidneys and spleen. Five of these birds were 

 killed and had been dissected at once. 



This Bacillus pyocyaneus was. as the inoculation experiments show, cap- 

 able of producing typical croupous and diphtheritic membranes in mouth and 

 eyes ; it was the cause of severe tumors in the submucous, or subcutaneous tis- 

 sue, the contents of which were firm, cheesy and yellowish-white. It produced 

 purulent conjunctivitis ; blindness ; purulent panophthalmia: inflammation of 

 the lungs ; and hard cheese-like exudations in the bronchia] tubes. In a word, 

 it produced symptoms identical with those of roup. 



The second virulent form of bacteria isolated seems to be a new organ- 

 ism. We have named it B. cacosmus and s'hall also refer to it as the roup 

 bacillus. It was in great numbers in the croupous membranes under the 

 tongue of hens i and 9 ; in the cheesy material of the tumor on the head of hen 

 5. as well as in the purulent conjunctival secretion ; in the nasal secretion of h n 

 4 ; in the purulent masses which closed the lacrymal duct of hen 21 ; and was 

 also found in a number of other birds affected with roup. 



