20 



Gelatine Streak Cultures. Along the needle track, a thm, grey, shining 

 growth appears, under which the gelatine soon becomes furrowed. The groove 

 sinks deeper into the gelatine, and the liquefied gelatine and the bacteria flow 

 as a turbid mass to the bottom of the tube. 



Gelatine Stick Cultures. After 24-28 hours, a homogeneous, fine, grey 

 band forms along the line of puncture. At tihe same time, on the surface of 

 the gelatine, a smooth, fine covering grows, and, after two or three days, the 

 gelatine becomes liquefied slowly around the puncture, spreads gradually over 

 the whole surface of the gelatine, and then the liquefaction becomes stratiform. 

 The liquefied gelatine is turbid, but never ropy. On the surface oi the solid 

 gelatine, a copious grey sediment settles. After two or three weeks, when 

 about 2-3 of the gelatine has become liquefied, growth ceases. The liquid 

 mass gives a very strong alkaline reaction, and has a very disagreeable putrid 

 sm 11. 



Agar Plate Cultures. At ,57 degrees C, in 24 hours the surface colonies 

 appear as smooth, gleaming points, whicih grow quickly into round or irregular 

 shaped masses. The centre becomes somewhat thicker and greyer. After 

 two days, they are three to five mm. in diameter. The whole colony has an 

 iridiscent lustre. Deep colonies appear as grey points. Seen through the 

 microscope, they are round, or somewhat irregular, clear, and darker at the 

 margin. 



Agar Streak Cultures. The culture spreads over the whole surface, and 

 forms a smooth, faintly gleaming, thin, grey cover, wlhich appears blue when 

 the light falls on it. The condensation water becomes turbid and grev. On 



/ e 



Fig;. 22.— From the broncliial exudate No l(i. 

 ('. A motile infusorium with ciliated crown. 



Fig. 23.— Bodies from the slim.\' pus from the Regio 

 olfactoria, or hen No. 23. V. Ciliated cells witli 

 swollen nuclei, ji. A foreign body, perhaps of 

 vegetable ori"in. 



agar, with 5 per cent, glycerine, the gro-wth is the same, only much slower. 



Bouillon Cultures. In 24 hours, the bouillon is uniformly turbid. The 

 reaction is unchanged, and a grey sediment forms which diffuses uniformly 

 when the test-tube is shaken. After 4 or 5 days, a thin, grey pellicle appears 



