12 



Inoculation of Freshly Gathered Vegetables. 



In all the following experiments, the vegetables were obtained 

 fresh from the garden. These vegetables were thoroughly washed in 

 running tap water and then, by means of sterilized cool knife, slices 

 were cut and placed in Petri dishes. These slices were immediately 

 inoculated by rubbing a platinum loop, (which had been charged with 

 a bouillon culture) over their surface. In all cases, uninoculated 

 slices of the different vegetables were also kept in order to check 

 any contamination from germs growing on the surface, or from those 

 which might develop from insufficent care in the preparation of the 

 slices. In no cases did such uninoculated .slices decay or rot. 



All the cultures were kept at room temperature, which varied 

 from 2U-2S° C. 



Cauliflower. The whole plant, after rinsing, was cut down the 

 centre and placed in a large dish, and then inoculated. In two days, 

 the stem and flower had discolored to a dirty brown, and softening 

 extended downwards about 20 m. m. There was a very disagreeable 

 smell. In 7 days, the whole of the plant was completely rotted 

 could be cut down and across with a platinum needle and the dirty 

 brown color was darker. Gas bubbles were present in all the decayed 

 parts, 



Cabbage. Cabbage plants, treated like the cauliflower, under- 

 went the same change and in 7 days there was a complete softening 

 of the whole plant, with the production of a very bad odour. 



Turnip (White). In two days a whitish wet growth spread 

 over the surface of the slice. There was a pale brown discoloration 

 of the infected part. The rot extended downwards to a depth of 10 

 m. m. In 5 days, complete softening had occured. The smell was 

 slightly disagreeable. 



Turnip (Swede). Decay in the yellow turnip was usually 

 slower than in the white, but depended largely on the amount of 

 moisture present. Where the turnip was very moist, decay advanced 

 rapidly; but on drier surfaces decay was slower, and at times no 

 growth took placj. 



There was considerable exudation of water on the inoculated 

 part and abundant brown-black growth which softened to a depth of 

 4 m.m. After two days growth, gas bubbles were present. Fre- 

 quently, a whitish moist growth was noticed instead of the brownish- 

 , bhick, due either to difference of water content or to difference of 



