16 



Stains. The bacillus from gelatine culture stains well with 

 gentian violet, not so well taken from agar. Carbolfuchsin gives good 

 results, for cover glass preparations and also for sections of diseased 

 tissues. It stains slowly with methelene bluie. In three minutes the 

 bacilli are only very faintly colored. 

 It does not accept Gram's Stain. 



Cultural Characters. 

 Bouillon at 28 C. In 24 hours the culture was very turbid, no 

 pellicle and heavy sediment. In 48 hours the turbidity increased. 

 The sediment was heavier and llocculent masses w^ere deposited on 

 the sides of the tube. A ring formed at the surface. In three da^^s a 

 pellicle formed which settled on slight disturbances. In six days the 

 pellicle and ring on undisturbed tubes were heavier. 

 Media remained turbid (4 weeks). 



In cabbage bouillon with 1 per cent, of peptone the organism 

 grew very well, and produced heavy turbidity and cojjious sediment 

 in 24 hours, a slight ring formed at the surface on the fifth day, 

 otherwise there was no change. 



Gelatine. On plate cultures of nutrient gelatine the colonies 

 were visible to the naked eye in 24 hours. They were punctiform 

 and round. With § objective they appeared round, homogenous, with 

 weak refraction and entire edges. In 48 hours the surface colonies 

 were 2 m.m. in diameter, liquefying, round, greyish white in color aad 

 with a ring in the centre composed of deposited bacilli. Under the 

 microscope (f obj.) they were round, coarsely granular, the centre was 

 grumose, and the edges slightly ciliate. Deep colonies were consider- 

 ably smaller, less than 1 mm. in diameter, round, internal structure 

 moruloid, edges of some colonies were entire, others with effused 

 growth. There was considerable variation. 



In three days the surface colonies were from 3-5 m. m. in 

 diameter round, greyish color, liquefaction shallow, often with one or 

 two concentric rings. Under the microscope the edges appeared 

 ciliate, the centre moruloid, and the rest of the colony granular. The 

 bacterial masses might be seen in motion. 



The deep colonies were smaller with darker centre and ciliate 

 edge, the fringe being longer and more wavy and interwoven than in 

 the surface colonies. 



In 4 days the colonies were larger in size otherwise there was no 

 change. 



