17 



In stich cultures at 20° C. there was a white growth along the 

 line of puncture in 24 hours. Slight liquefaction at the surface, 

 I m.m. in diameter, along the line of inoculation the growth was 

 slightly heavier. In 4 days the liquefied area reached the sides of 

 the test tube and thereafter liquefaction was stratiform. 



There was often considerable difference in the rate of lique- 

 faction, at times the whole tube might become liquefied, at other 

 times only the half. 



Wort gelatine. Stich cultures. The organism grew very well in 

 this medium, witli shallow pit liquefaction, followed by stratiform 

 liquefaction to a depth of 5 m.m. in 7 days (20' C.) Growth stopped 

 when about half the mc dium was liquefied. 



Whey gelatine stich cultures. There was a crateriform depression 

 12 m.m. in diameter, with deposition of a flocculent mass in the 

 centre of the pit in 24 hours. In 48 hours liquefaction had extended 

 to the sides of the tubes and downwards to a depth of 2 m.m. at the 

 sides and 3 m.m. in the centre. In three days the liquefied portion 

 was 5 m.m. deep and growth ceased when 9 m.m. deep. A few gas 

 bubbles appeared in the gelatine at some distance from the line of 

 puncture 



Agar. On agar plates at 28° C. colonies were not characteristic. 

 Surface colonies spread very fast, as thin grey expansions, which 

 varied greatly in shape. Deep colonies were dense, punctiform, 

 round, or elliptical ; in fact, there seemed every variety of shape 

 Agar slope cultures at 28' grew very rapidly over the surface as a 

 moist, thin, whitish growth, slightly opalescent by transmitted light. 

 There was considerable deposit of the bacilli in the condensation 

 water. The growth became more massive with age, otherwise there 

 was no change. 



Garho-hydrate agars. Slope and shake cultures were made in 

 ao-ars containing 2% of the following carbo-hydrates : Glycerine, 

 saccharose, lactose, glucose, and maltose. The media was made from 

 Liebicr's Extract of Meat, reaction neutral. Check cultures were 

 made in agar without carbohydrates, no gas formed in these. 



In crlycerine agar the growth "vas more abundant and whiter than 

 on plain agar. No gas in the shake culture and heaviest growth on 

 the surface. 



In saccharose agar amount of growth exceeded that on plain 

 agar. In shake culture a few gas bubbles were present. 

 2 Bull. 137 



