19 



The viability of the organism in milk was as follows : Cultures, 

 25 days old, living; 35 days old, living ; two months old, dead. 



Litmus Milk at 25"* C. In 2* hours the color compared with the 

 control tubes was appreciably different. In 48 hours, the color was 

 lighter, between lilac and livid (Saccardo 48 and 49), the milk was 

 thick but not coagulated. In three days, the milk coagulated into a 

 soft even curd with about 10 m. m, of whey on the surface and a few 

 gas bubbles in the coagulum. Colour lilac (Saccardo 48\ In four 

 days, the curd had shrunk leaving a clear whey on the surface. The 

 curd when shaken separated into flaky masses and gas bubbles were 

 fairly numerous through the curd on the surface. The color of the 

 curd at the bottom of the tube was white, the upper portion, lilae. 

 On the fifth day, the whey was slightly tinged with color ; the lower 

 half of the curd was white and the upper half, lilac. On the eighth 

 day, there was only a small red ring of color at the top of the curd. 

 On the twelfth day, the lilac color again returned. 



Blood Serurn at 25' C. On blood serum good growth occurred, — 

 first, as a moist slightly spreading growth, later becoming heavier, 

 more opaque and opalescent by transmitted light, The condensation 

 water was turbid. Slight liquefaction was visible on the 8th day, 

 and in 21 days most of the sloped surface became liquid and no further 

 chantje occurred. The bacilli from blood serum shewed banded and 

 bipolar staining with carbol-fuchsin. 



Egg Media. (Dorset's method). Good growth occurred on egg 

 media, spreading over the entire surface. No liquefaction occurred in 

 24 days and the growth was not characteristic. 



Dunhams Solution at 25' C. In Dunham's solution there was 

 slight growth ; and uniform turbidity in 24 hours, the cloudiness 

 increased and a slight sediment formed which became flocculent in 

 four days. Eight-day cultures gave a very slight indol re-action, 

 while in 15-day cultures the re-action wa« more marked. 



In Dunham's solution with Rosolic acid (in the same proportion 

 as used by Jones), the salmon pink colour almost entirely faded in 24 

 hours. In 48 hours, the tubes were quite decolorised and remained 

 so three months. Rosolic acid bouillon was decolorised in the 

 same way. This change shewed the formation of acid. 



Synthetic Media. In TLschinsky's medium there was turbidity 

 with some sediment in 24 hours at 25°C. A slight pellicle formed in 

 48 hours, and the body of the media became more turbid with increase 



