20 



of sediment. In seven days there was a thick pellicle and heavy 

 sediment, but the body of the liquid was almost clear. In 15 days 

 the pellicle gradually sank, the body of the liquid was pale yellow, 

 and there was a copious sediment. 



In Fermi's medium, there was slight turbidity in the upper third 

 of the medium and a verv slight sediment in 24 hours at 25°C. A 

 thin pellicle formed in 48 hours and the "top of the liquid was very 

 cloudy. On shaking, the pellicle produced turbidity throughout the 

 entire medium. The growth at four, seven and fifteen days resembled 

 growth in Uschinsky's medium. 



In Lager Beer Wort, 12.2 Ball, good growth occured, at tirst 

 turbid and with considerable sediment. The liquid was several 

 shades lighter in color, and a few gas bubbles were seen. In three 

 days, the wort was quite clear, with heavy fine sediment and no 

 pellicle. No further change occurred. 



Cooked Vegetables. Generally speaking, the growth on cooked 

 vegetables was abundant, but the softening action of the organism on 

 the cooked vegetables was not always as marked as its action on raw 

 vegetables ; in other words the production of cystase was more marked 

 when the organism was placed upon slices of raw vegetables. 



Potato prepared according to Roux's method, reaction slightly 

 acid to litmus. In 24 hours there was a moist, shiny, spreading 

 growth distinguishable from the potato by the glistening appearance. 

 The growth became more massive and on the drier slices the growth 

 was more waxy looking and straw colored. No further change 

 occurred and the potato slice was slightly softened, it could never be 

 cut quite through with the platinum needle. 



On potato cylinders prepared by immersing half the slice in 

 water, the growth was moist and spreading. The water was at first 

 turbid with much sediment, consisting of particles of softened potato^ 

 pure white in colour. In seven days the liquid became yellow in 

 colour and the sediment was pure white. Gas bubbles were also 

 present. 



The immersed portion of the cylinder was softened, but in 

 twenty days the core above the water was still firm and could not be 

 cut with the platinum wire, a control test on raw potatoes from the 

 same source caused complete softening of the tissues in three days. 



In other tests of potatoes there were minor differences — Thus the 

 growth would be dirty yellow, or honey yellow in colour, and the 



