21 



moist and glistening appearance on some potatoes would be changed 

 to a dull waxy looking growth. 



Differences in the rate and extent of softening also occurred. 

 In all some 60 tests were made on potatoes. 



On cooked carrot at 28"C, there was a moist spreading growth 

 with complete softening in three days. 



On cooked sugar beet there was a flat, shiny, moist growth ; gas 

 bubbles were present, and the cylinder was completely softened in 

 four days. 



On cooked beet-root there was a whitish spreading growth, the 

 beet was discoloured (brown-green), and there was a white, slightly 

 raised moist growth, with complete softening. 



On cooked onion there was a moist, dirty white growth, the 

 onion was completely softened and fell to pieces. The odor was foul 

 and nauseating. 



Temperature relations. The optimum was about 30" C; there 

 was fast growth at 25° to 28°. Good growth occurred at 20' and at 

 37.5' C. the growth was better than at 20 C. 



The maximum temperature was in the neighborhood of 42° C. 

 Tne minimum temperature was in the neighborhood of 5° C. 

 Thermal death 'point. The thermal death point was determined 

 by Sternberg's method. The temperature of the bath during the 

 time of exposure was varied about .25 of a degree. A temperature of 

 55° C. for 10 minutes was the thermal death point of the organism. 



Relation to free oxygen. The aerobic growth was better than 

 the anaerobic, but the organism grew in the closed arm of fermenta- 

 tion tubes, and in deep stich cultures. 



Agar, potato, gelatine slope and litmus milk cultures were grown 

 for eight days in a Novy jar in an atmosphere of hydrogen. 



There was slight growth limited to the needle track on the agar 

 slope ; slight growth but no liquefaction of the gelatine slope culture ; 

 very slight growth on the potato ; slight growth and change of colour 

 in milk, but no coagulation. The cultures when taken out of the 

 Novy jar grew vigorously. The bacilli from the agar culture were 

 rather shorter, averaging about 1.5/', in length. 



Nitrate hroth at 25° C. In nitrate broth growth was better than 

 in Dunham's solution. The media becomes turbid with tirst a fine 

 and later a fiocculent sediment. No pellicle formed. 



