TISSUE CULTURES. 21 



ing to Costantin and Matriichot," Van Tiegheiii (1876) produced the 

 mycelium of Coprinus in pure culture. Later, Brgfeld " accom- 

 plished the same result with many species of Coprinus. and also with 

 Armillaria meUea. Costantin has also published a number of brief 

 papers, or announcements, of successful cultures upon artificial media 

 of the mycelium of several fleshy fimgi. ^osidefi Agm^icus campestris^ 

 he has grown the mycelium of Amanita rubescens, Armillaria mellea^ 

 Gollyhia velutipes^ Lepiota procera^ Marasmius oreades, Trieholoma 

 nudum^ Pleurotus ostreatus^ Pholiota aegerita^ Coprinus comatus, 

 Polyporus tnheraster^ P. frondosus^ Hydnum coralloides^ Morchella 

 esculentu, and perhaps a few others. He has also grown to maturity 

 sporojDhores of Agarlcus campestris^ Coprinus comatus^ and Tri- 

 choloma nudum. Unfortunately, Costantin seldom indicates the 

 substratum upon which his cultures were made. Falck ^ reports 

 having produced in culture the sporophores of CoUyhia velutipes, 

 Phlehia ?nerismoides, Hypholoma fa^scicidare., Chalymotta campanu- 

 lata., and Coprinus ephemerus in his studies upon the connection of 

 oidial stages with perfect forms of the Basidiomycetes. In the work 

 of the writer so far no special attempt has been made to obtain the 

 sporophores of the fungi cultivated except in the case of Agaricus 

 campestris. Nevertheless, the following species have fruited in pure 

 culture upon the media indicated : 



Medium. 



Agaricus campestris Manure. 



Agaricus fabaceus Manure. 



Agaricus amygdalinus Manure. 



Armillaria mellea Beans. 



Bovistella ohiensis Soil. 



Calvatia cyathiforme Soil. 



Calvatia rubro-flava Soil. 



Cortinarius sp Soil. 



Coprinus comatus Leaves. 



Coprinus fimetarius Leaves. 



Coprinus solstitialis (?) Leaves, etc. 



Daedalia quercina Leaves, etc. 



Hydnum coralloides Beans. 



Lycoperdon wrightii Soil. 



Pleurotus ostreatus Beans and manure. 



Pleurotus ulmarius Manure. 



In some in.stances the sporophores have been minute, owing to the 

 small quantity of the culture medium. 



a Brefeld. O. Tenters, aus d. C}esammtgel)ipte d. Mykologie. S. f), 10. 



6 Falclv, R. Die Cultur der Oidien und die Riickfiibrung in die tiohere Frucbr- 

 form bei den Basidiomyceteu. Cobn's Beitriige /ur Biologie der I'tiauzeu, 8: 

 307-346 (Pis. 12-17). 



