16 



MUSHROOM GROWING AND SPAWN MAKING. 

 Table I. — Extent of germination — Continued. 



No. 



11 

 12 



13 

 14 



15 



16 



17 

 18 



19 

 20 



21 



22 



33 

 24 

 25 

 26 



27 

 28 



29 



30 



31 



32 



33 



34 

 35 



36 



37 



38 



39 



40 



41 

 42 



43 



Media. 



i per cent MgH4(P04)o 



i per cent MgH4(P04)2 with bou- 

 illon. 



f per cent MgHP04. 



i per cent MgHP04 in bouillon . 



Iper centMg(NH4)P04 



I per cent Mg(NH4)P04 in bou- 

 illon. 



I per cent MgK{NH4)P04---. 



Iper cent MgK(NH4)H2(P04) 

 bouillon. 



lain 



I per cent (NH4)oC4H40o 



I per cent (NH4)2C4H40,i in bou- 

 illon. 



I per cent magnesium lactophos- 

 phate. 



i per cent magnesium lactophos- 

 phate in bouillon. 



iper cent Ca2Ho(P04)2 



i per cent Ca..H.r(P04 )•> in bouillon 



i per cent KCHOo ...'. __.. 



I per cent MgHPOij 



\ per cent MgHPOs in bouillon, _ 



iper cent MgK(NH4)H2(P04)2 

 in mushroom decoction. 



i per cent KH2PO4 in mushroom 

 decoction. 



J per cent K0HPO4 in mushroom 

 decoction. 



J per cent NaoHP04 in mush- 

 room decoction. 



I per cent ( NH4)2HP04 in mush- 

 room decoction. 



i per cent MgHP04 in mush- 

 room decoction. 



do_ 



i per cent Mg(NH4)P04 in mush- 

 room decoction. 



i^ per cent (NH4)2C4H40oinmush- 

 room decoction. 



i per cent magnesium lactophos- 

 phate in mushroom decoction. 



i per cent Ca2H2(P04)2 in mush- 

 room decoction. 



i per cent KCHOo in mushroom 

 decoction. 



i per cent MgHPOa in mush- 

 room decoction. 



Decoction of mushrooms . . _ 



After 3 days 



Living tissue of mushroom in 

 mushroom decoction. 



....do 



10 spores 



fa— 1 spore . . . 

 [6— None 



None 



a — 10 spores . 



None 



2 spores . 



Few spores 

 do 



fa — None . 



\6— None.. 



10 spores 



do.... 



do.... 



None 



10 spores . 



None 



10 spores . 



do.... 



do.... 



None. 



.do. 



Few spores . 

 1 or 2 spores 

 Few spores . 



10-50 per cent . 

 Few spores 



2 per cent . . . 



10 spores 



5-8 per cent . 

 2-3 per cent . 



^1-2 per cent 



f o — 2 per cent 



{b — Very few spores 



a — Few spores « 



6— None'' _.. 



(o— Few spores" 



16— None!* 



After 5 days. 



.50 per cent. 

 3 per cent. 

 None. 

 (Do. 

 1 1 per cent. 

 Germinated spores badly 



injured. 

 None. 

 As before; injured. 



5 per cent. 

 Do. 



Few spores. 



10 per cent. 



As before; injured. 



5-10 per cent. 



2-5 per cent. 



1-2 per cent. 

 Injured. 

 1-2 per cent. 

 10-50 per cent. 

 1 per cent. 

 1-2 per cent. 



None. 



Do. 



Injured. 



Few spores. 



2-5 per cent. 



10-20 per cent. 

 Contaminated. 



Contaminated; 5 per 



cent, but injured. 

 3-5 per cent; injured. 



10 per cent. 



10-20 per cent. 



0—5 per cent. 

 6— Contaminated 

 2 per cent. 

 1-2 per cent. 

 12 spores. 



As before. 

 None. 



{1 



" In this cell the tissue developed a new growth. 

 '' No growth from tissue introduced. 



On account of the fact that magnesium phosphite and magnesium 

 potassium ammonium phosphate had in most cases proved to be 

 stimuli for germination, experiments were next made to determine 

 the efficiency of these salts on various media, as indicated in the 

 table on the following page. 



