CHLOROPHYLLS C AND d, BACTEKIO- AND PROTOCHLOKOPHYLL 017 



Table 21. Ill 



ISOMERIZATION AND OtHER REACTIONS OF CHLOROPHYLLS d AND a " 



Chlorophyll d' 



It 

 Chlorophyll d 



Isochlorophyll d' 



Gochlorophyll d 



CH-Mgl 



Pheophytin d 



KOH 



KOH 



— Isopheophytin d 



KOH 



KOH 



681 m^ 698 m;i 658 m^ 636 m^ 



CHjCOOH CHjCOOH 



672 m^ 691 Wfi 



660 m/i 672 m/i 



HCI 



HCI 



HCI 



HCI 



SSOmjii 



" After Maiming and Strain (1943). 



Chlorophyll u' 



Chlorophyll a 



,. 

 HCI CHjMgl 



Pheophytin a 



KOH 



KOH 



662 m^ 649 my. 

 CHjCOOH 



667 m^ 652 m^ 



HCI Ihci 



— f — 



667 m^ 



0.20 



Fig. 21.6. Relative absorption curve 

 of green pigment from bacterium Spi- 

 rillum rubrum in methanol (after French 

 1937). 0.2 ml. moist cells extracted in 

 the dark at 0° with 5.2 ml. absolute 

 methanol Extract kept in dark and 

 measured at room temperature with 

 very weak light. A similar curve was 

 given by Vermeulen, Wassink and 

 Reman (1937) for alcoholic extract from 

 Chromaiium (major peak at 790 m/n, 

 minor peaks at 705, 600, 510, 470 and 

 440 mM). 



600 800 



WAVE LENGTH, m/i 



lies in the near infrared and has not yet been measured precisely; its gen- 

 eral shape is shown by figure 21.(). According to this figure, the absorp- 

 tion spectrum of bacteriochlorophyll contains three main bands — one in the 

 infrared (770 m/i in methanol, shown also in fig. 21.21), one in the orange 

 (005 m/x) and one in the violet (~400 ni/i). One may assume {cf. below, 

 page 622) that the two latter bauds are analogous to the red and the blue- 



