COLLOIDAL AND ADSORBED CHLOROPHYLL 



653 



figures for the blue-violet bands are so high as to call for a recheck (see 

 chapter 22, page 705, for the position of the blue-violet bands in the living 

 cells). Eisler and Portheim (1922) and Noack (1927) stated that the ab- 

 sorption spectra of chlorophyll adsorbates on proteins are "similar to those 

 of the living cells," but gave no figures. 



640 680 72C 



WAVE LENGTH, m>i 



640 680 720 



WAVE LENGTH, m^ 



2.U 



(of) I. Cells in distilled water 

 2. Colloidal extract in 

 fresh egg albumen 



ENERGY, e.v. 



1.9 1.8 



T 



T 



"T 



17 



(e) 1. Cells in distilled water 

 2. Alcoholic extract 



ENERGY, e.v. 

 19 "1.8 1.7 



T 



640 680 720 



WAVE LENGTH, m^ 



640 680 720 



WAVE LENGTH, m>t 



■(c) Knop's culture nfiedium 



640 »- 680 720 



WAVE LENGTH, m^A 



Fig. 21 .29. Comparative absorption .spectra of cell suspensions and pigment extracts of 

 Chlorella in different media (after Katz and Wassink 1939). Curve 1, cells; curve 2, ex- 

 tracts. See page 65G. 



Absorption curves ai'O available for "natural" chlorophyll-protein 

 colloids extracted from leaves, algae and bacteria. Smith (1938, 1941) 

 gave an absorption curve of the crude extract from spinach leaves, which 

 contains broken chloroplasts or grana, and another curve for the same ex- 

 tract clarified by digitonin (r/. fig. 21.28, curves A and B). The maximum 

 of curve A is at 678 m;u, that of curve B at 675 m/x. 



