FLUORESCENCE SPECTRUM OF CHLOROPHYLL 



741 



Figure 23.1 A shows the appearance of the fluorescence spectrum of 

 chlorophyll on a panchromatic plate, and figure 23. IB, on an infrared- 



Wave length, m/i 



Reference spectrum (He + Hg) 

 ' Absorption 



Chloroptiyll a 



Fluorescence 



Reference spectrunn (He + Hg) 

 ' Absorption 



Chlorophyll a ■ 



Fluorescence 

 with self- 

 absorption 

 Exciting light ( \ < 470 m/i) 

 Calibration spectrum (Nernst lamp) 

 Reference spectrum (He + Hg) 



o 

 o 



Wave length, m^ 



Reference spectrum (He + Hg ) 

 Absorption 



Chlorophyll 

 a + b 



Fluorescence ■ 



Chlorophyll o (Fluorescence) 

 Reference spectrum (He + Hg) 

 Chlorophyll ( Absorption 



a + b I Fluorescence 

 Chlorophyll (Fl. with self-obs.) 

 Exciting light ( X< 470 m/i) 

 Calibration spectrum (Nernst lamp) 

 Reference spectrum (He + Hg) 



Fig. 23.1 A. Fluorescence spectra of chloroj)!!}'!! (a at top and a + b below) in ether on 

 panchromatic plates (after Dhere and Fontaine, 1931). 



sensitive plate. Fig. 23.2 shows spectrophotometric curves of the fluores- 

 cence of chlorophylls a and 6 in ether, determined by Zscheile and Harris 



