Reprinle<i from llie Hutamcai. GAZETIK45: 232-253, April 1908 



FURTHER STUDIES OF SOLUTION TENSION AND 



TOXICITY IN LIPOLYSIS- 



Raymond H. Pond 



This paper is really a continuation of an earlier one* which supplied 

 data showing that the toxicity of certain salts in lipolysis docs not vary 

 inversely as the decomposition tension of those salts. As my conclu- 

 sion disagrees with that of Mathews,^ McGuigan,'» and Caldwell, ^ 

 and as Berg and Gies^ found that the nature of the zymolytc is an 

 imj)ortant factor in ])eptolysis and tryptolysis, it seemed desirable to 

 determine whether the same general relation found by me for the tox- 

 icity of several nitrates in the lipolysis of ethyl butyrate would also hold 

 in the case of some other ester. Ethyl acetate was selected as the 

 zymolyte because its saponification is a monomolecular reaction, 

 because acetic acid is a close homologuc of butyric acid, and because 

 Loevenhart and Peirce' found that the hydrolysis of ethyl acetate 

 is inhibited to a greater extent by sodium fluorid than that of ethyl 

 butyrate. They also expressed the view that the inhibiting effect of 

 sodium fluorid is dependent upon the acid radical from which the 

 ester is derived, while the alcohol arising in the saponification is a 

 comparatively indifferent factor. 



Since the same general procedure has been followed here as in the 

 preceding investigation, many of the details given before will be omitted 



' From the laboratories of the New York Botanical Garden. 



2 Pond, Raymond H., Solution tension and toxicity in lipolysis. Amer. Jour. 

 Physiology 19:258-283. 1907. Contributions from the New York Botanical Gar- 

 den 94. 



3 Mathews, A. P., The relation between solution tension, atomic volume, and 

 the physiological action of the elements. Amer. Jour. Phy.siology 10:290-323. 1904. 



4 McGuiGAN, Hugh M., The relation between the decomposition tension of 

 salts and their antifermentative properties. Amer. Jour. Physiology 10:444-451. 

 1904. 



s Caldwell, J. .S., The effects of toxic agents upon the action of bromelin. Bot. 

 Gazette 39:409-419. 1905. 



6 Berg, W. N., and Gies, Wm. J., Studies of the effects of ions on catalysis, with 

 particular reference to peptolysis and tryptolysis. Jour. Biol. Chem. 2:489-546. 1907. 



7 Loevenhart, A. S., and Peirce, Geo., The inhibiting effect of sodium fluorid 

 on the action of lipase. Jour. Biol. Chem. 2?57^4i3. 1907. 



Botanical Gazette, vol. 45 1 Z'^H [232 



