34^ Ibervillea Sonorae 



tuber was apparently normal in all respects. The mass of each tuber was 

 kept separate on the assumption that possibly the abnormal specimen 

 would show different toxic results from those of the normal tuber; also 

 with the idea that perhaps the toxicity ascribed to Ibervillea Sonorce in 

 general was due to special poisonous principles in such abnormal tubers 

 and that particular effort should be made to determine this possibility. 

 The rinds were also hashed. Each product was bottled and placed in a 

 cold room which was kept constantly at a temperature of about — 5° C. 



The following quotations from our records of this series of experiments 

 will suffice to show the results obtained. 



Sixth experiment with normal tuber. Weight of dog, 25.7 kilos.' 



1st day. Sixteen grams of tuber were administered in balls of meat at 

 1 1 a.m. In the afternoon a feverish and restless condition was observed. 

 This was very much less marked during the evening. Pronounced diar- 

 rhea occurred between midnight and 8 a.m. 2d day. By 10 a.m. the con- 

 dition of the dog was apparently very close to normal. Twenty-four grams 

 of tuber were given in meat at 10.30 a.m. The feverish condition reap- 

 peared unmistakably at 1 145 p.m. Vomiting occurred at intervals be- 

 tween 5 and 9:45 p.m. No defecation occurred during the day, at the 

 end of which the dog was apparently normal, ^d day. Thirty- two grams 

 of tuber were administered with the regular diet at 10:50 a.m. At 12 :3o 

 p.m. the animal became feverish and uncomfortable. Three hours later 

 very marked diarrhea occurred. About 6 p.m. a considerable portion of 

 the food given in the morning, including a fairly large proportion of meat, 

 was vomited. Between 7 and 8 p.m. marked diarrhea occurred. From 

 this time until the next morning, recovery was rapid. The dog was appar- 

 ently normal at the end of that period, /fih day. The dose of tuber was 

 again increased and 40 grams were given with the usual diet between 

 II a.m. and i p.m. At 1:40 p.m. the first signs of sickness appeared. 

 Between 4:15 p.m. and 5:20 p.m. very marked diarrhea occurred. This 

 was followed by rapid recovery and the dog was nearly normal four hours 

 later, ^th day. The animal refused to eat the meat balls containing the 

 tuber and so was allowed to fast until the sixth day. 6th day. The dog 

 again refused the meat balls. They were given forcibly. He received 

 50 grams of tuber at noon. The feeding was followed by a five-hour 

 period of feverishness and restlessness. This was succeeded by very 

 marked diarrhea. By midnight the dog had practically recovered. He 

 remained well. The same animal was used in the seventh experiment. 



Seventh experiment; with abnormal, i. e., "spotted" tuber, and with the 

 rind. About a month and a half after the completion of the preceding 

 experiment, the dog of that experiment, which showed no subsequent ill 

 effects of the treatment described above, was subjected to dosage with the 

 abnormal tuber. Diet and method of dosage were the same as in the 

 sixth experiment. 



' Daily diet — hashed meat, cracker meal, lard and water, with bone- ash. 

 A preliminary period of five days established normal conditions. 



