OF INSECTIVOROUS PLANTS 455 



experiments. On the fifth day distinct evidences were noted of in- 

 cipient digestion of the protein in all eight experiments; this digestion 

 was more marked on the ninth day, and still more marked on the twelfth 

 day. 



Each pitcher did not always contain sufficient liquor to permit a 

 complete set of experiments on all six of the agar media which contained 

 suspended proteins. However, a general tendency existed that, if the 

 micro-organisms present in the liquor grew on one of these media, they 

 grew on all of the media, and usually exerted a proteolytic action on all 

 the proteins. 



A spar agin rosolic acid agar. This medium was used in three series 

 of experiments. In the first series which included seven experiments, 

 colonies had developed by the fifth day, and a red (alkaline) color had 

 been imparted to the medium. By the fourteenth day, the medium had 

 become yellow in color (acid in reaction). 



The second series consisted of eight experiments. Colonies appeared 

 on all the plates by the third day. The entire medium next became alka- 

 line in reaction; this change had occurred on from the third to the fifth 

 day. While the colonies themselves remained alkaHne, the medium 

 finally became acid in reaction; this change had taken place in over one- 

 half of the experiments by the ninth day, and in the remaining experi- 

 ments by the twelfth day. 



The third series included seven experiments, in six of which good 

 growth of the bacteria and an alkaline reaction of the medium were 

 apparent by the third day. The medium had become acid in reaction 

 in one of these experiments by the tenth day. In the seventh experi- 

 ment of this series, growth had not occurred by the third day, but both 

 colonies and the alkaline reaction of the medium had developed by the 

 tenth day. The liquor from this series of seven pitchers was also sown 

 on glycocoll rosolic acid agar, acetamide rosolic acid agar, and anmionium 

 lactate rosolic acid agar; the results are given below. 



Glycocoll rosolic acid agar. In six experiments, good growth of the 

 bacteria had occurred, and an alkaline reaction had been imparted to 

 the entire medium at the end of three days; in one of these experiments, 

 about one -half the total area of the agar had become acid in reaction 

 by the tenth day. In the seventh experiment, bacterial growth did 

 not occur. 



Acetamide rosolic acid agar. In six experiments, good growth of the 

 bacteria and an alkaHne reaction of the medium were noted by the third 



