33° 



BOTANICAL GAZETTE 



[NOVEMBER 



solution, in which i cc. represents 3 . 1 rag. of copper. The corre- 

 sponding equivalents of the different sugars expressed in milligrams 

 were found in the Munson-Walker table. The weight of sugar 

 found divided by the material used gives the amount of sugar con- 

 tained in 1 gm. of material. 



The soluble carbohydrates are in F x and F,. The reducing 

 sugar was first determined. The non-reducing sugar was obtained 

 by subtracting the reducing sugar from the total sugar by hydro- 

 chloric acid hydrolysis at 67-69 C. for 10 minutes. 



The insoluble carbohydrates are in F 3 . They consist essentially 

 of colloidal polysaccharides, the greater part of which was starch. 

 The polysaccharides were determined by the Fehling solution after 

 acid hydrolysis for 2 . 5 hours with a reflex condenser. 



TABLE III 



The carbohydrates in the green plant 



The percentage of the different carbohydrates of various organs 

 estimated at different times throughout the growth period is tabu- 

 lated in table III. Curves showing the changes in different sugar 

 content in these organs are given in figs. 6-8. These curves show 

 that in the roots the reducing sugars remain constant, while the 



