324 



BOTAMCAL GAZETTE 



[novembes 



In general the amino N varies directly with the insoluble protein; 

 with a high protein content, high amino N; and low protein con- 

 tent, low amino X. 



TABLE II 

 The nitrogen compounds in the green plant 



Materia! 



Total X 



Nitrates N0 3 . . . 



Nitrate N 



Amino X 



Insoluble X 



Insoluble protein 

 Soluble protein. . 



Total X 



Nitrates N0 3 . . . 



Nitrate N 



Amino X 



Insoluble X 



Insoluble protein 

 Soluble protein. . 



Total N 



Nitrates X0 3 . . . 



Xitrate X 



Amino X 



Insoluble X 



Insoluble protein 

 Soluble protein. . 



Roots 



Stems 



Branches 



June 3 collection (1-4 inches) 



3-33 



1.77 



0.40 

 1 .09 

 1 .62 

 10.18 10.25 



8.24 



3-33 

 i-93 



43 



1 13 

 1 52 



319 

 o.99 

 o. 22 

 043 

 i-74 



3-21 



1 .04 



°- 2 3 

 043 

 i-73 



8.1: 



10. 92 10.80 

 7.67 7.92 



June 20 collection (6-8 



inches) 



i.»5 



Xone 



Xone 

 o. 29 

 0.86 

 5 40 

 5-92 



1.88 



Xone 



None 



o. 29 



0.91 



5-72 



6. 10 



2-39 

 67 

 38 

 19 

 95 

 86 

 28 



2. 21 



1. 75 



0.40 

 o. 19 

 0.97 

 6. 10 

 5-4i 



July 8 collection (20 inches) 



3.21 

 4.41 

 O.99 

 0.63 

 I . 29 

 8.IO 

 6.16 



3.12 



4-47 

 1 .02 

 0. 70 

 1. 14 

 7.17 

 6.05 



5 04 

 12.50 

 2.85 

 0.48 

 1 .01 



6-35 

 7.42 



Leaves 



25 

 36 

 31 

 97 

 21 



20. 20 

 458 



.27 

 .40 

 •32 

 .96 

 23 

 ■3° 

 •52 



456 



None 



None 



1.38 



3-66 



None 

 None 

 1 37 

 3-59 



23.00 22.6 

 5.66 5.78 



4.85 



0.25 

 0.06 



1 44 

 3 29 



4.80 

 o. 246 

 0.06 

 1.42 

 3.21 



20.62 20.08 

 9.42 9.62 



Insoluble protein. — The insoluble protein was calculated from 

 nitrogen of the insoluble fraction (F 3 ). The insoluble protein is 

 given in table II, and curves showing fluctuation during the growing 

 season are given in figs. $-5. The insoluble protein falls and then 

 rises again at maturity in the root, while in the leaves the fluctua- 

 tion is in the opposite direction (see curves). In the stem the 

 decline is in the early stage from 10.89 P er cent (June 3) to 6.03 per 

 cent (June 20). From that time on the curve is almost a straight 

 horizontal line; therefore the rate of synthesis of the insoluble 

 protein must have been keeping pace with the growth of the stem, 



