

A CHEMICAL ANALYSIS OF SUDAN GRASS SEED 



CONTRIBUTIONS FROM THE HULL BOTANICAL LABORATORY 258 



F. M. S C H E HTZ 

 (WITH ONE FIGURE) 



The method as here outlined was originally taken from the 

 methods of Waldemar Koch, 1 who employed it in the analysis of 

 brain tissues. The method was then further modified by F. C. 

 Koch, 2 of the department of physiological chemistry, University 

 of Chicago, where the work was chiefly on animal tissues. The 

 method was again modified to meet the needs of plant tissues. 



Outline of method 

 Dry or germinating seeds 



Soluble portion (Fj + F 2 ) Insoluble residue (F 3 ) 



Ether soluble portion (Fj) Water, alcohol soluble portion (F 2 ) 



Fraction i (Fi) is the ether soluble portion; fraction 2 (F 2 ) 

 is the portion soluble in alcohol or water; and fraction 3 (F 3 ) is 

 the portion which is insoluble in ether, water, or alcohol. The 

 dry seeds were ground finely before making the extraction, while 

 the germinating seeds were ground in a mortar as finely as possible. 

 The material was then placed in the extraction cups and extracted 

 for 4 hours. A i-hour extraction with ether was then made and 

 the ether extract was added to the alcohol extract. The residue 

 was dried, ground in a mortar, and then a water extraction was 

 made. This water extraction and the residue was then made up to 

 70 per cent alcohol and again extracted with 95 per cent alcohol for 

 1 2 hours. In some cases this extraction was found to be insufficient, 



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1 Koch, WALDEMAR, Methods for the quantitative chemical analysis of anuria ^ 

 tissues. Archives Neurology and Psychiatry 4:11. 1909; also Jour. Amer. Chem ZZ. 

 Soc. 31: 1329-1364. 1909. »~» 



2 Outline for the analysis of tissues as prepared by F. C. Koch. C«3 



Botanical Gazette, vol. 69] I?- 2 cr* 



.V) 



