1920] 



ROSE, KRAYBILL, Sr ROSE— OXIDASE ACTIVITY 



226 



TABLE V 



Effect of o. 10 N MnCl 2 and K 2 S0 4 on oxidation of pyrogallol by precipitated 



OXIDASE FROM BOTH HEALTHY AND DISEASED APPLE BARK; TEMPERATURE 



29.5-30.2° C. 



Time ok reading 



June 21 



i-45 



2 .00 



2-15 



2.30 



2.45 



3 .00 



3-15 



3 30 



3-45 

 4.00 



4-15 

 4-3o 

 4-45 

 June 22 

 8.00 



00 

 07 

 08 

 08 

 08 

 oS 



15 

 15 



iS 



20 

 20 



23 



28 



Healthy 



Check MnCla K,S() 4 



0-53 



OO 

 08 

 10 

 13 

 13 

 10 

 II 

 08 

 09 

 08 



o 



o. 



o. 



o, 



o. 



o. 



o 



o 



o 



o 



0.08 



0.09 



0.18 

 0.28 



000 



Oil 



O. 21 



0.23 



o. 27 

 o. 25 



0.28 



0.30 



035 

 0.34 

 0.37 



0.38 

 043 



0.58 



Check 



O.OO 

 O.17 



0.37 



O.42 

 O.48 

 O.SO 



56 



6S 



70 



79 



87 

 88 

 98 



Diseased 



I.24 



MnCl. 



OO 



IS 



29 



21 

 25 

 23 

 24 

 26 



o. 



o, 



o. 



o 



o 



o 



o 



o 



o. 29 



° 3 1 



0.34 

 0.35 



0.40 



0.63 



KaS0 4 



0.00 



0.15 



0.30 



o.33 

 o.43 

 0.48 



054 

 0.58 

 0.63 

 0.69 

 0.78 

 0.78 

 o 93 



1.28 



Fig. 5. — Effect of MnCl 2 and K 2 S0 4 on the oxidation of pyrogallol on precipitated 

 oxidase from both healthy and diseased bark: A (diseased), K 2 S0 4 +bark and pyro- 

 gallol; B (diseased), control (bark and pyrogallol); C (diseased), MnCl 2 +bark and 

 pyrogallol; D (healthy), K 2 S0 4 +bark and pyrogallol; E (healthy), control (bark 

 and pyrogallol); F (healthy), MCnl 2 +bark and pyrogallol. 



